A sandwiched-culture technique for evaluation of heterologous protein production in a filamentous fungus

被引：0
|
作者：
Asgeirsdóttir, SA [1 ]
Scholtmeijer, K [1 ]
Wessels, JGH [1 ]
机构：
[1] Univ Groningen, Plant Mol Biol Lab, Biomol Sci & Biotechnol Inst, NL-9751 NN Haren, Netherlands
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1999年 / 65卷 / 05期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous proteins. Expressing the ABH1 hydrophobin of Agaricas bisporus in A. niger; we showed that low production levels of the heterologous protein are probably due to extracellular proteolytic degradation of the protein.
引用
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页码：2250 / 2252
页数：3
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