A rise in the hydrolysis activity of Candida rugosa lipase caused by pressurized treatment with supercritical carbon dioxide

被引：0
|
作者：
Yan, H [1 ]
Noritomi, H [1 ]
Nagahama, K [1 ]
机构：
[1] Tokyo Metropolitan Univ, Dept Appl Chem, Grad Sch Engn, Hachioji, Tokyo 1920397, Japan
来源：
KOBUNSHI RONBUNSHU | 2001年 / 58卷 / 12期
关键词：
Candida rugosa lipase; supercritical carbon dioxide; hydrolysis activity;
D O I：
10.1295/koron.58.674
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
The hydrolysis activity of Candida rugosa lipase (lipase OF) treated with supercritical CO., was measured. The activity of the lipase, which was treated for one hour at 308.15 K and 19.61 MPa, is approximately 2.5 times higher than that of the untreated lipase. Changes in the content of proteins in the lipase and also in the size of the lipase particle after treatment were investigated through fluorescence emission spectra meter and scanning electron microscopy respectively. The size reduction of particles and the purification of the enzyme might be the main reasons for the increase in Candida rugosa lipase's activity.
引用
收藏
页码：674 / 678
页数：5
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