Aptamer-based label-free detection of PDGF using ruthenium(II) complex as luminescent probe

被引：21
|
作者：
Babu, E. [1 ]
Singaravadivel, S. [1 ]
Manojkumar, P. [2 ]
Krishnasamy, S. [2 ]
Kumar, G. Gnana [1 ]
Rajagopal, S. [1 ]
机构：
[1] Madurai Kamaraj Univ, Sch Chem, Dept Phys Chem, Madurai 625021, Tamil Nadu, India
[2] Madurai Kamaraj Univ, Sch Biotechnol, Dept Genet Engn, Madurai 625021, Tamil Nadu, India
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2013年 / 405卷 / 21期
关键词：
Ru(II) complex; Aptamer; PDGF; Biosensor; Luminescence; GROWTH-FACTOR; DNA; BINDING;
D O I：
10.1007/s00216-013-7118-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We report a simple, cost-effective, and label-free detection method, consisting of a platelet-derived growth factor (PDGF) binding aptamer and hydrophobic Ru(II) complex as a sensor system for PDGF. The binding of PDGF with the aptamer results in the weakening of the aptamer-Ru(II) complex, monitored by luminescence signal. A substantial enhancement in the luminescence intensity of Ru(II) complex is observed in the presence of aptamer due to the hydrophobic interaction. Upon addition of PDGF, the luminescence intensity is decreased, due to the stronger interaction between the aptamer and PDGF resulting in the displacement of Ru(II) complex to the aqueous solution. Our assay can detect a target specifically in a complex medium such as the mixture of proteins, at a concentration of 0.8 pM.
引用
收藏
页码：6891 / 6895
页数：5
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