Occurrence of Carbapenemase-Producing Klebsiella pneumoniae in Bat Guano

被引：0
|
作者：
Gharout-Sait, Alima [1 ]
Touati, Abdelaziz [1 ]
Ahmim, Mourad [2 ]
Brasme, Lucien [3 ,4 ]
Guillard, Thomas [3 ,4 ]
Agsous, Amir [1 ]
de Champs, Christophe [3 ,4 ]
机构：
[1] Univ Bejaia, FSNV, Lab Ecol Microbienne, Bejaia 06000, Algeria
[2] Univ Bejaia, FSNV, Lab Ecol & Environm, Bejaia 06000, Algeria
[3] CHU Reims, Hop Robert Debre, Lab Bacteriol, Virol Hyg Hosp, Ave Gen Koenig, Reims, France
[4] Univ Reims, Inserm UMR S 1250 P3Cell, SFR CAP Sante, Reims, France
来源：
MICROBIAL DRUG RESISTANCE | 2019年 / 25卷 / 07期
关键词：
OXA-48; KPC-3; Klebsiella pneumoniae; bat guano; Algeria; SPECTRUM-BETA-LACTAMASE; ANTIBIOTIC-RESISTANCE; RAPID DETECTION; ENTEROBACTERIACEAE; PLASMID; KPC; IDENTIFICATION; SALMONELLA; BACTERIA; GENE;
D O I：
10.1089/mdr.2018.0471
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The aim of this study was to screen for the presence of carbapenemase-producing Enterobacteriaceae (CPE) isolates from bat guano in Bejaia, Algeria. Guano samples (n = 110) were collected in Aokas's cave, Bejaia, Algeria, between March and May 2016. Samples were plated on MacConkey agar supplemented with ertapenem (0.5 mg/L) and vancomycin (32 mg/L). The isolates were identified and antimicrobial susceptibility was determined using disk diffusion method. Carbapenemase, extended spectrum beta-lactamases, plasmid-mediated AmpC, and plasmid-mediated quinolone resistance genes were studied using PCR and sequencing. Clonal relatedness was studied using multilocus sequence typing (MLST). Two CPE isolates were identified as Klebsiella pneumoniae. PCR and sequencing identified the blaOXA-48 in one K. pneumoniae strain (CS34) and blaKPC-3 in the other strain (CS63). K. pneumoniae CS63 was found to carry bla(TEM-1) and aac(6 ')-Ib genes. The MLST showed that K. pneumoniae CS63 was assigned to ST512, whereas K. pneumoniae CS34 belonged to ST1878. This is the first description of CPE from bats' guano.
引用
收藏
页码：1057 / 1062
页数：6
相关论文
共 50 条
  • [31] Assessment of Antimicrobial Combinations for Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae
    Hirsch, Elizabeth B.
    Guo, Beining
    Chang, Kai-Tai
    Cao, Henry
    Ledesma, Kimberly R.
    Singh, Manisha
    Tam, Vincent H.
    JOURNAL OF INFECTIOUS DISEASES, 2013, 207 (05): : 786 - 793
  • [32] A Threat of the Klebsiella Pneumoniae Carbapenemase-Producing Strains Among Transplant Recipients
    Mlynarczyk, G.
    Kosykowska, E.
    de Walthoffen, S. Walter
    Szymanek-Majchrzak, K.
    Sawicka-Grzelak, A.
    Baczkowska, T.
    Pazik, J.
    Durlik, M.
    Ciszek, M.
    Paczek, L.
    Chmura, A.
    Mlynarczyk, A.
    TRANSPLANTATION PROCEEDINGS, 2011, 43 (08) : 3135 - 3136
  • [33] PROGNOSTIC FACTORS OF CRITICALLY ILL PATIENTS WITH KLEBSIELLA PNEUMONIAE CARBAPENEMASE-PRODUCING
    Santos, Maria Lucia
    Taminato, Monica
    Ramos, Gabriel
    Delgado, Artur
    Ferreira, Juliana
    Carvalho, Werther
    Monteiro, Ana Cristina
    CRITICAL CARE MEDICINE, 2020, 48
  • [34] Within-patient plasmid dynamics in Klebsiella pneumoniae during an outbreak of a carbapenemase-producing Klebsiella pneumoniae
    Stohr, Joep J. J. M.
    Verweij, Jaco J.
    Buiting, Anton G. M.
    Rossen, John W. A.
    Kluytmans, Jan A. J. W.
    PLOS ONE, 2020, 15 (05):
  • [35] Carbapenemase-Producing Klebsiella pneumoniae Infections in Diabetic and Nondiabetic Hospitalized Patients
    Pattolath, Athira
    Adhikari, Prabha
    Pai, Vidya
    CUREUS JOURNAL OF MEDICAL SCIENCE, 2024, 16 (01)
  • [36] Molecular characterization of Klebsiella pneumoniae carbapenemase-producing isolates in southern Brazil
    Ribeiro, Vanessa Bley
    Andrade, Leonardo Neves
    Linhares, Adriano Rostirolla
    Barin, Juliana
    da Costa Darini, Ana Lucia
    Zavascki, Alexandre P.
    Barth, Afonso Luis
    JOURNAL OF MEDICAL MICROBIOLOGY, 2013, 62 : 1721 - 1727
  • [37] Molecular epidemiology of carbapenemase-producing Klebsiella pneumoniae in Gauteng South Africa
    Salvador-Oke, Kafilat T.
    Pitout, Johann D. D.
    Peirano, Gisele
    Strydom, Kathy-Anne
    Kingsburgh, Chanel
    Ehlers, Marthie M.
    Ismail, Arshad
    Takawira, Faustinos T.
    Kock, Marleen M.
    SCIENTIFIC REPORTS, 2024, 14 (01):
  • [38] Trends in Carbapenemase-producing Klebsiella pneumoniae, Taiwan, 2012-2015
    Chiu, Sheng-Kang
    Ma, Ling
    Chan, Ming-Chin
    Fung, Chang-Phone
    Wu, Tsu-Lan
    Chuang, Yin-Ching
    Lu, Po-Liang
    Wang, Jann-Tay
    Lin, Jung-Chung
    Yeh, Kuo-Ming
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2017, 50 : S116 - S116
  • [39] Outbreak of colistin-resistant carbapenemase-producing Klebsiella pneumoniae in Tunisia
    Mansour, Wejdene
    Haenni, Marisa
    Saras, Estelle
    Grami, Raoudha
    Mani, Yosra
    Khalifa, Anis Ben Haj
    el Atrouss, Souhail
    Kheder, Mohamed
    Hassen, Mohamed Fekih
    Boujaafar, Noureddine
    Bouallegue, Olfa
    Madec, Jean-Yves
    JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2017, 10 : 88 - 94
  • [40] Carbapenemase-producing Klebsiella pneumoniae with two different gene expression patterns
    Werner, G.
    Grensemann, J.
    Helmers, A.
    Wappler, F.
    Sakka, S. G.
    MINERVA ANESTESIOLOGICA, 2015, 81 (11) : 1273 - 1274
12345