Wheat alkylresorcinols protect human retinal pigment epithelial cells against H2O2-induced oxidative damage through Akt-dependent Nrf2/HO-1 signaling

被引：3
|
作者：
Wang, Ziyuan [1 ]
Hao, Yiming [1 ]
Wang, Yu [1 ]
Liu, Jie [1 ]
Yuan, Xiaoping [2 ]
Sun, Baoguo [1 ]
Wang, Jing [1 ]
机构：
[1] BTBU, China Canada Joint Lab Food Nutr & Hlth Beijing, Beijing 100048, Peoples R China
[2] China Grain Reserves Corp, Dept Sci & Technol, Beijing 100039, Peoples R China
来源：
FOOD & FUNCTION | 2019年 / 10卷 / 05期
关键词：
CHOLESTEROL EXCRETION; VITAMIN-A; ACTIVATION; STRESS; NRF2;
D O I：
10.1039/c8fo02564a
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The protective effect of wheat alkylresorcinols (ARs) on human retinal pigment epithelium cells (ARPE-19) against oxidative stress and the possible underlying mechanism were investigated in this study. The results showed that ARs significantly inhibited 300 M H2O2-induced ARPE-19 cell damage and reactive oxygen species (ROS) generation by 19% and 32%, respectively. Moreover, ARs treatment increased NF-E2-related factor 2 (Nrf2) signaling activation, which was evidenced by increased transcription of anti-oxidant responsive genes GCL, NQO1 and HO-1. Knockdown of Nrf2 through targeted siRNA alleviated ARs-mediated HO-1 transcription, and almost abolished ARs-mediated cytoprotection against H2O2 induced cell damage. Further studies showed that the protective effect of ARs was dependent on Akt activation. Taken together, these results demonstrated that ARs could protect ARPE-19 cells from oxidative stress induced cell damage possibly through Akt dependent Nrf2/HO-1 signaling.
引用
收藏
页码：2797 / 2804
页数：8
相关论文
共 50 条
  • [31] Activation of Nrf2/HO-1 antioxidant signaling correlates with the preventive effect of loganin on oxidative injury in ARPE-19 human retinal pigment epithelial cells
    Choi, Yung Hyun
    GENES & GENOMICS, 2023, 45 (03) : 271 - 284
  • [32] Novel lignans from Syringa pinnatifolia and protective effect against H2O2-induced oxidative injury through regulating the expression of Nrf2/ HO-1 in H9c2 cells
    Yang, Guodong
    Liu, Changxin
    Chen, Panlong
    Jiao, Shungang
    Sun, Jingjing
    Liang, Yana
    Cui, Lingwen
    Wang, Tao
    Gao, Xiaoli
    Zhang, Qingying
    Chai, Xingyun
    BIOORGANIC CHEMISTRY, 2024, 152
  • [33] Orientin-mediated Nrf2/HO-1 signal alleviates H2O2-induced oxidative damage via induction of JNK and PI3K/AKT activation
    Xiao, Qingfei
    Piao, Rongli
    Wang, Hongyue
    Li, Chenhao
    Song, Liangsong
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 118 : 747 - 755
  • [34] Punicalagin Protects Human Retinal Pigment Epithelium Cells from Ultraviolet Radiation-Induced Oxidative Damage by Activating Nrf2/HO-1 Signaling Pathway and Reducing Apoptosis
    Clementi, Maria Elisabetta
    Sampaolese, Beatrice
    Sciandra, Francesca
    Tringali, Giuseppe
    ANTIOXIDANTS, 2020, 9 (06)
  • [35] HO-1 protects human melanocytes from H2O2-induced oxidative stress via Nrf2-ARE pathway
    Jian, Zhe
    Li, Kai
    Li, Chunying
    Zhang, Ying
    Liu, Ling
    Gao, Tianwen
    JOURNAL OF DERMATOLOGY, 2010, 37 : 116 - 117
  • [36] Curcumin prevents high glucose damage in retinal pigment epithelial cells through ERK1/2-mediated activation of the Nrf2/HO-1 pathway
    Bucolo, Claudio
    Drago, Filippo
    Maisto, Rosa
    Romano, Giovanni Luca
    D'Agata, Velia
    Maugeri, Grazia
    Giunta, Salvatore
    JOURNAL OF CELLULAR PHYSIOLOGY, 2019, 234 (10) : 17295 - 17304
  • [37] Salvianolic acid A protects RPE cells against oxidative stress through activation of Nrf2/HO-1 signaling
    Zhang, Hui
    Liu, Yuan-yuan
    Jiang, Qin
    Li, Ke-ran
    Zhao, Yu-xia
    Cao, Cong
    Yao, Jin
    FREE RADICAL BIOLOGY AND MEDICINE, 2014, 69 : 219 - 228
  • [38] Ursonic Acid Ameliorates H2O2-Induced Oxidative Damage in PC12 Cells and Prolonged the Lifespan in C. elegans by Activating MAPKs/Nrf2/HO-1 Signaling Pathway
    Wang, Jiahui
    Yang, Jia
    Lu, Jia
    Lu, Jingshi
    Ling, Bai
    Song, Yan
    Chen, Guangtong
    MOLECULAR NEUROBIOLOGY, 2025, : 6598 - 6611
  • [39] Rutin modified selenium nanoparticles reduces cell oxidative damage induced by H2O2 by activating Nrf2/HO-1 signaling pathway
    Zhu, Mengyu
    Zhang, Yang
    Zhang, Chi
    Chen, Lei
    Kuang, Ying
    JOURNAL OF BIOMATERIALS APPLICATIONS, 2023, 38 (01) : 109 - 121
  • [40] Forsythiaside Protected H9c2 Cardiomyocytes from H2O2-Induced Oxidative Stress and Apoptosis via Activating Nrf2/HO-1 Signaling Pathway
    Yang, Zhicai
    Ning, Xiaokang
    Zhang, Ying
    INTERNATIONAL HEART JOURNAL, 2022, 63 (05) : 904 - 914
12345