Deficiency of phospholipase C-γ1 impairs renal development and hematopoiesis

被引：0
|
作者：
Shirane, M
Sawa, H
Kobayashi, Y
Nakano, T
Kitajima, K
Shinkai, Y
Nagashima, K
Negishi, I
机构：
[1] Nippon Roche Res Ctr, Dept Mol Oncol, Kamakura, Kanagawa 2478530, Japan
[2] Nippon Roche Res Ctr, Dept Prod Res, Kamakura, Kanagawa 2478530, Japan
[3] Hokkaido Univ, Grad Sch Med, Lab Mol & Cellular Pathol, CREST,JST,Kita Ku, Sapporo, Hokkaido 0608638, Japan
[4] Osaka Univ, Res Inst Microbial Dis, Dept Mol Cell Biol, Suita, Osaka 5650871, Japan
[5] Kyoto Univ, Inst Virus Res, Dept Cell Biol, Sakyo Ku, Kyoto 6068507, Japan
[6] Gunma Univ, Sch Med, Dept Dermatol, Maebashi, Gumma 3718511, Japan
来源：
DEVELOPMENT | 2001年 / 128卷 / 24期
关键词：
PLC-gamma; 1; chimeric mice; renal dysplasia; hematopoiesis; in vitro differentiation;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Phospholipase C-gamma1 (PLC-gamma1) is involved in a variety of intracellular signaling via many growth factor receptors and T-cell receptor. To explore the role of PLC-gamma1 in vivo, we generated the PLC-gamma1-deficient (plc-gamma1(-/-)) mice, which died of growth retardation at embryonic day 8.5-9.5 in utero. Therefore, we examined plc-gamma1(-/-) chimeric mice generated with plc-gamma1(-/-) embryonic stem (ES) cells for further study. Pathologically, plc-gamma1(-/-) chimeras showed multicystic kidney due to severe renal dysplasia and renal tube dilation. Flow cytometric analysis and glucose phosphate isomerase assay revealed very few hematopoietic cells derived from the plc-gamma1(-/-) ES cells in the mutant chimeras. However, differentiation of plc-gamma1(-/-) ES cells into erythrocytes and monocytes/macrophages in vitro was observed to a lesser extent compared with control wild-type ES cells. These data suggest that PLC-gamma1 plays an essential role in the renal development and hematopoiesis in vivo.
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收藏
页码：5173 / 5180
页数：8
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