Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae

被引：12
|
作者：
Daniel, JA
Yoo, JY
Bettinger, BT
Amberg, DC
Burke, DJ
机构：
[1] Univ Virginia, Med Ctr, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA
[2] SUNY Upstate Med Univ, Dept Biochem & Mol Biol, Syracuse, NY 13210 USA
来源：
GENETICS | 2006年 / 172卷 / 01期
关键词：
D O I：
10.1534/genetics.105.047662
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Synthetic genetic analysis was improved by eliminating leaky expression of the HIS3 reporter and gene conversion between the HIS3 reporter and his3 Delta 1. Leaky expression was eliminated using 3-aminotriazole and gene conversion was eliminated by using the Schizosaccharomyces pombe his5(+) gene, resulting in a 5- to 10-fold improvement in the efficiency of SGA.
引用
收藏
页码：709 / 711
页数：3
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