Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae

被引：12
|
作者：
Daniel, JA
Yoo, JY
Bettinger, BT
Amberg, DC
Burke, DJ
机构：
[1] Univ Virginia, Med Ctr, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA
[2] SUNY Upstate Med Univ, Dept Biochem & Mol Biol, Syracuse, NY 13210 USA
来源：
GENETICS | 2006年 / 172卷 / 01期
关键词：
D O I：
10.1534/genetics.105.047662
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Synthetic genetic analysis was improved by eliminating leaky expression of the HIS3 reporter and gene conversion between the HIS3 reporter and his3 Delta 1. Leaky expression was eliminated using 3-aminotriazole and gene conversion was eliminated by using the Schizosaccharomyces pombe his5(+) gene, resulting in a 5- to 10-fold improvement in the efficiency of SGA.
引用
收藏
页码：709 / 711
页数：3
相关论文
共 50 条
  • [1] High-throughput isolation of Saccharomyces cerevisiae RNA
    Dye, BT
    Hao, LH
    Ahlquist, P
    [J]. BIOTECHNIQUES, 2005, 38 (06) : 868 - +
  • [2] A high-throughput method for Saccharomyces cerevisiae (yeast) ionomics
    Danku, John M. C.
    Gumaelius, Luke
    Baxter, Ivan
    Salt, David E.
    [J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2009, 24 (01) : 103 - 107
  • [3] An Evaluation of High-Throughput Approaches to QTL Mapping in Saccharomyces cerevisiae
    Wilkening, Stefan
    Lin, Gen
    Fritsch, Emilie S.
    Tekkedil, Manu M.
    Anders, Simon
    Kuehn, Raquel
    Michelle Nguyen
    Aiyar, Raeka S.
    Proctor, Michael
    Sakhanenko, Nikita A.
    Galas, David J.
    Gagneur, Julien
    Deutschbauer, Adam
    Steinmetz, Lars M.
    [J]. GENETICS, 2014, 196 (03) : 853 - +
  • [4] High-throughput analysis of adaptation using barcoded strains of Saccharomyces cerevisiae
    Fasanello, Vincent J.
    Liu, Ping
    Botero, Carlos A.
    Fay, Justin C.
    [J]. PEERJ, 2020, 8
  • [5] High-throughput measurement of recombination rates and genetic interference in Saccharomyces cerevisiae
    Raffoux, Xavier
    Bourge, Mickael
    Dumas, Fabrice
    Martin, Olivier C.
    Falque, Matthieu
    [J]. YEAST, 2018, 35 (06) : 431 - 442
  • [6] High-throughput transformation of Saccharomyces cerevisiae using liquid handling robots
    Liu, Guangbo
    Lanham, Clayton
    Buchan, J. Ross
    Kaplan, Matthew E.
    [J]. PLOS ONE, 2017, 12 (03):
  • [7] A synthetic suicide riboswitch for the high-throughput screening of metabolite production in Saccharomyces cerevisiae
    Lee, Sang-Woo
    Oh, Min-Kyu
    [J]. METABOLIC ENGINEERING, 2015, 28 : 143 - 150
  • [8] High-Throughput Screening of Inhibitory Compounds on Growth and Ethanol Production of Saccharomyces cerevisiae
    Jeffery A. Wood
    Valerie C. A. Orr
    Luis Luque
    Vivek Nagendra
    Franco Berruti
    Lars Rehmann
    [J]. BioEnergy Research, 2015, 8 : 423 - 430
  • [9] A rapid, high-throughput method for quantitative determination of ethanol tolerance in Saccharomyces cerevisiae
    Tran, Tina M. T.
    Stanley, Grant A.
    Chambers, Paul J.
    Schmidt, Simon A.
    [J]. ANNALS OF MICROBIOLOGY, 2013, 63 (02) : 677 - 682
  • [10] High-Throughput Biochemical Fingerprinting of Saccharomyces cerevisiae by Fourier Transform Infrared Spectroscopy
    Kohler, Achim
    Bocker, Ulrike
    Shapaval, Volha
    Forsmark, Annabelle
    Andersson, Mats
    Warringer, Jonas
    Martens, Harald
    Omholt, Stig W.
    Blomberg, Anders
    [J]. PLOS ONE, 2015, 10 (02):
12345