HPLC-ESI-MS/MS analysis of phenolics and in vitro antioxidant activity of Epilobium angustifolium L.

被引:14
|
作者
Deng, Li-Qing [1 ]
Zhou, Si-Yu [1 ]
Mao, Jin-Xin [1 ]
Liu, Shuang [1 ]
Lan, Xiao-Zhong [2 ]
Liao, Zhi-Hua [3 ]
Chen, Min [1 ]
机构
[1] Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence & Real Time Anal, Minist Educ, Chongqing, Peoples R China
[2] XiZang Agr & Anim Husb Coll, Nyingchi, Tibet, Peoples R China
[3] Southwest Univ, Sch Life Sci, Chongqing, Peoples R China
关键词
Epilobium angustifolium L; antioxidant activity; phenolics; HPLC-ESI-MS/MS;
D O I
10.1080/14786419.2017.1344659
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The aerial parts of Epilobium plants are widely used as folk medicine and food around the world. The present study was aimed to investigate the antioxidant activities and active chemical constituents from Epilobium angustifolium L. The results revealed that the EtOAc extract, rich in phenolic compounds and flavonoids (16.81 +/- 0.67g GAE/100g extract and 4.95 +/- 0.21g QE/100g extract, respectively), possessed significantly antioxidant activities in reducing power, DPPH radical scavenging activity, ABTS radical scavenging activity and highly in inhibiting lipid peroxidation activity. Simultaneously, active fractions F to H from EtOAc extracts showing potent in vitro antioxidant activities also contained high content of total phenolic and flavonoid. Twenty-eight compounds were identified as phenolic compounds and flavonoids by LC-MS/MS. The results illustrate that the E. angustifolium L., which is rich in phenolics, could be used as a natural resource of antioxidant ingredient. [GRAPHICS] .
引用
收藏
页码:1432 / 1435
页数:4
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