Assessment of fungal contamination in moldy homes: Comparison of different methods

In an effort to better understand the relationship between different fungal sampling methods in the indoor environment, four methods were used to quantify mold contamination in 13 homes with visible mold. Swab, fungal spore source strength tester (FSSST), and air samples (total of 52 samples) were analyzed using both the microscopic (total spore count) and culture-based (CFU count) enumeration techniques. Settled dust samples were analyzed for culturable fungi only, as the microscopic enumeration was restricted by the masking effect. The relationships between the data obtained with the different sampling methods were examined using correlation analysis. Significant relationships were observed between the data obtained from swab and FSSST samples both by the total counting (r = 0.822, p < 0.05) and by the CFU counting (r = 0.935, p < 0.01). No relationships were observed between air and FSSST samples or air and settled dust samples. Percentage culturability of spores for each sampling method was also calculated and found to vary greatly for all three methods (swab: 0.03% to 63%, FSSST 0.1% to > 100%, air: 0.7% to 79%). These findings confirm that reliance on one sampling or enumeration method for characterization of all indoor mold source might not provide an accurate estimate of fungal contamination of a microenvironment. Furthermore, FSSST sampling appears to be an effective measurement of a mold source in the field, providing all upper bound estimate of potential mold spore release into the indoor air. Because of the small sample size of this study, however further research is needed to better understand the observed relationships in this study.