Deep and Clear Optical Imaging of Thick Inhomogeneous Samples

被引：50

作者：

Jorand, Raphael ^{[1
,2
]}

Le Corre, Gwenaele ^{[1
,2
]}

Andilla, Jordi ^{[3
]}

Maandhui, Amina ^{[1
,2
]}

Frongia, Celine ^{[1
,2
]}

Lobjois, Valerie ^{[1
,2
]}

Ducommun, Bernard ^{[1
,2
,4
]}

Lorenzo, Corinne ^{[1
,2
]}

机构：

[1] Univ Toulouse, ITAV UMS3039, Toulouse, France

[2] CNRS, ITAV UMS3039, Toulouse, France

[3] Inst Ciences Foton, ICFO, Barcelona, Spain

[4] CHU Toulouse, Toulouse, France

来源：

PLOS ONE | 2012年 / 7卷 / 04期

关键词：

ADAPTIVE OPTICS; MICROSCOPY;

D O I：

10.1371/journal.pone.0035795

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Inhomogeneity in thick biological specimens results in poor imaging by light microscopy, which deteriorates as the focal plane moves deeper into the specimen. Here, we have combined selective plane illumination microscopy (SPIM) with wavefront sensor adaptive optics (WAO). Our WAOSPIM is based on a direct wavefront measure using a Hartmann-Shack wavefront sensor and fluorescent beads as point source emitters. We demonstrate the use of this WAOSPIM method to correct distortions in three-dimensional biological imaging and to improve the quality of images from deep within thick inhomogeneous samples.

引用

页数：9

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