Complete genome organization of American hop latent virus and its relationship to carlaviruses

被引：6
|
作者：
Eastwell, Kenneth C. [1 ]
Druffel, Keri L. [2 ]
机构：
[1] Washington State Univ, Dept Plant Pathol, IAREC, Prosser, WA 99350 USA
[2] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA
来源：
ARCHIVES OF VIROLOGY | 2012年 / 157卷 / 07期
关键词：
PURIFICATION;
D O I：
10.1007/s00705-012-1312-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The complete genomic sequence of American hop latent virus (AHLV; genus Carlavirus) was determined. The genome consists of 8,601 nucleotides plus a 3'-polyadenylate tail. The genome encompasses six potential open reading frames (ORF) in the positive sense, and their organization is typical of other carlaviruses. Analysis of the coat protein coding sequence at both the nucleic acid level and the amino acid level indicates that AHLV is only remotely related to the other carlaviruses known to infect common hop. Polyclonal antibodies were produced against the bacterially expressed coat protein of AHLV. These antibodies differentiated between AHLV and other carlaviruses of hop.
引用
收藏
页码：1403 / 1406
页数：4
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