Isolation and characterization of microsatellite loci in Branchiostoma belcheri Gray (Amphioxus)

被引：0
|
作者：
Li, Z. B. [1 ,2 ]
Huang, Y. S. [1 ,2 ]
Shangguan, J. B. [1 ,2 ]
Ning, Y. F. [1 ,2 ]
Yi, Y. [1 ,2 ]
Dai, G. [1 ,2 ]
机构：
[1] Jimei Univ, Coll Fisheries, Xiamen, Peoples R China
[2] Fujian Prov Key Lab Marine Fishery Resources & Ec, Xiamen, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2015年 / 14卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Branchiostoma belcheri Gray; FIASCO; Conservation; Microsatellite;
D O I：
10.4238/2015.August.28.5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Branchiostoma belcheri Gray is a second-class, nationally protected protochordate in China. We developed 10 novel polymorphic sites in B. belcheri, which were examined using a population of 30 wild individuals from Xiamen, China. The polymorphism information content ranged between 0.141 and 0.681, and the number of alleles varied from 2 to 5. The expected and observed heterozygosities varied between 0.1528 and 0.6920, and between 0.1429 and 0.5000, respectively. These novel microsatellite markers will facilitate the genetic analysis and protection of wild B. belcheri individuals, and the possible re-stocking of the species in the long-term.
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页码：10224 / 10227
页数：4
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