Prediction and Identification of B-Cell Epitopes on Goose Parvovirus Capsid Protein

被引：0
|
作者：
Yu, Tian-fei [1 ,3 ]
Li, Ming [2 ]
Guo, Yuan [2 ]
Song, Guang-jun [2 ]
Li, Rong-xiu [3 ]
Xu, Ai-zhang [3 ]
Shao, Shu-li [1 ]
机构：
[1] Qiqihar Univ, Coll Life Sci & Agr Forestry, 42 Wenhua St, Qiqihar 161006, Peoples R China
[2] Qiqihar Univ, Coll Comp & Control Engn, Qiqihar 161006, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Life Sci Biotechnol, Key Lab, Minist Educ Microbial Metab, Shanghai 200240, Peoples R China
来源：
2011 INTERNATIONAL CONFERENCE ON ENVIRONMENT SCIENCE AND BIOTECHNOLOGY (ICESB 2011) | 2011年 / 8卷
关键词：
goose parvovirus; capsid protein; prediction; identification; epitope; LINKED-IMMUNOSORBENT-ASSAY; VIRAL DISEASE; VIRUS; GOSLINGS; GEESE;
D O I：
10.1016/j.proenv.2011.10.045
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The capsid protein (VP) gene of goose parvovirus (GPV) was translated into amino sequence by Editseq. The secondary structure and B-cell epitopes of the deduced protein were predicted by Protean. Combining the results according to these methods, the VP had complicated secondary structure. There were several potential B-cell epitopes on VP. By means of overlapping peptides expressed in Escherichia coli in combination with Western blot, B-cell epitopes were identified on VP. The results showed virtual linear immunodominant epitopes, which were found in seven fragments covering amino acid residues 35-81, 111-161, 423-453, 462-491, 531-577, 616-669, 678-732. (C) 2011 Published by Elsevier Ltd. Selection and/or peer-review under responsibility of the Asia-Pacific Chemical, Biological & Environmental Engineering Society (APCBEES)
引用
收藏
页码：284 / 288
页数：5
相关论文
共 50 条
  • [41] Identification of recombination between Muscovy duck parvovirus and goose parvovirus structural protein genes
    Shen, Hongxing
    Zhang, Wen
    Wang, Hua
    Zhou, Yang
    Shao, Shihe
    ARCHIVES OF VIROLOGY, 2015, 160 (10) : 2617 - 2621
  • [42] Identification of B-cell Epitopes of Peanut Ara h 2
    Liao, Sumei
    Patil, Sarita U.
    Shreffler, Wayne G.
    Dreskin, Stephen C.
    Chen, Xueni
    JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2017, 139 (02) : AB255 - AB255
  • [43] Expression of goose parvovirus VP1 capsid protein by a baculovirus expression system and establishment of fluorescent antibody test to diagnose goose parvovirus infection
    K. Takehara
    T. Nakata
    K. Takizawa
    C. K. Limn
    K. Mutoh
    M. Nakamura
    Archives of Virology, 1999, 144 : 1639 - 1645
  • [44] Identification of recombination between Muscovy duck parvovirus and goose parvovirus structural protein genes
    Hongxing Shen
    Wen Zhang
    Hua Wang
    Yang Zhou
    Shihe Shao
    Archives of Virology, 2015, 160 : 2617 - 2621
  • [45] Expression of goose parvovirus VP1 capsid protein by a baculovirus expression system and establishment of fluorescent antibody test to diagnose goose parvovirus infection
    Takehara, K
    Nakata, T
    Takizawa, K
    Limn, CK
    Mutoh, K
    Nakamura, M
    ARCHIVES OF VIROLOGY, 1999, 144 (08) : 1639 - 1645
  • [46] IMMUNOGENICITY OF POLIOVIRUS B-CELL AND T-CELL EPITOPES PRESENTED BY HYBRID PORCINE PARVOVIRUS PARTICLES
    SEDLIK, C
    SARRASECA, J
    RUEDA, P
    LECLERC, C
    CASAL, I
    JOURNAL OF GENERAL VIROLOGY, 1995, 76 : 2361 - 2368
  • [47] Mapping of B-cell epitopes of hemagglutinin protein of rinderpest virus
    Renukaradhya, GJ
    Mitra-Kaushik, S
    Sinnathamby, G
    Rajasekhar, M
    Shaila, MS
    VIROLOGY, 2002, 298 (02) : 214 - 223
  • [48] Structural analysis of B-cell epitopes in antibody:protein complexes
    Kringelum, Jens Vindahl
    Nielsen, Morten
    Padkjaer, Soren Berg
    Lund, Ole
    MOLECULAR IMMUNOLOGY, 2013, 53 (1-2) : 24 - 34
  • [49] Mapping of B-cell epitopes in the nucleocapsid protein of Puumala hantavirus
    Lundkvist, Å
    Meisel, H
    Koletzki, D
    Lankinen, H
    Cifire, F
    Geldmacher, A
    Sibold, C
    Gött, P
    Vaheri, A
    Krüger, DH
    Ulrich, R
    VIRAL IMMUNOLOGY, 2002, 15 (01) : 177 - 192
  • [50] Identification and characterization of a common B-cell epitope on EIAV capsid proteins
    Hu, Zhe
    Chang, Hao
    Chu, Xiaoyu
    Li, Shuang
    Wang, Meiyue
    Wang, Xiaojun
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 100 (24) : 10531 - 10542
12345