Prediction and Identification of B-Cell Epitopes on Goose Parvovirus Capsid Protein

被引：0

作者：

Yu, Tian-fei ^{[1
,3
]}

Li, Ming ^{[2
]}

Guo, Yuan ^{[2
]}

Song, Guang-jun ^{[2
]}

Li, Rong-xiu ^{[3
]}

Xu, Ai-zhang ^{[3
]}

Shao, Shu-li ^{[1
]}

机构：

[1] Qiqihar Univ, Coll Life Sci & Agr Forestry, 42 Wenhua St, Qiqihar 161006, Peoples R China

[2] Qiqihar Univ, Coll Comp & Control Engn, Qiqihar 161006, Peoples R China

[3] Shanghai Jiao Tong Univ, Sch Life Sci Biotechnol, Key Lab, Minist Educ Microbial Metab, Shanghai 200240, Peoples R China

来源：

2011 INTERNATIONAL CONFERENCE ON ENVIRONMENT SCIENCE AND BIOTECHNOLOGY (ICESB 2011) | 2011年 / 8卷

关键词：

goose parvovirus; capsid protein; prediction; identification; epitope; LINKED-IMMUNOSORBENT-ASSAY; VIRAL DISEASE; VIRUS; GOSLINGS; GEESE;

D O I：

10.1016/j.proenv.2011.10.045

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The capsid protein (VP) gene of goose parvovirus (GPV) was translated into amino sequence by Editseq. The secondary structure and B-cell epitopes of the deduced protein were predicted by Protean. Combining the results according to these methods, the VP had complicated secondary structure. There were several potential B-cell epitopes on VP. By means of overlapping peptides expressed in Escherichia coli in combination with Western blot, B-cell epitopes were identified on VP. The results showed virtual linear immunodominant epitopes, which were found in seven fragments covering amino acid residues 35-81, 111-161, 423-453, 462-491, 531-577, 616-669, 678-732. (C) 2011 Published by Elsevier Ltd. Selection and/or peer-review under responsibility of the Asia-Pacific Chemical, Biological & Environmental Engineering Society (APCBEES)

引用

页码：284 / 288

页数：5

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