Genotoxic effects of 8-hydroxylquinoline in loach (Misgurnus anguillicaudatus) assessed by the micronucleus test, comet assay and RAPD analysis

被引：17
|
作者：
Nan, Ping [1 ]
Xia, Xiao-hua [1 ]
Du, Qi-yan [1 ]
Chen, Jian-jun [1 ]
Wu, Xiao-hua [1 ]
Chang, Zhong-jie [1 ]
机构：
[1] Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Henan, Peoples R China
来源：
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2013年 / 35卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Genotoxicity; Loch (Misgurnus anguillicaudatus); 8-Hydroxylquinoline; Micronucleus; Comet assay; RAPD; AMPLIFIED POLYMORPHIC DNA; TROUT SALMO-TRUTTA; STRAND BREAKS; CELLS; RESISTANCE; MUTATION; DETECT; DAMAGE;
D O I：
10.1016/j.etap.2013.02.005
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
This study was a preliminary step in evaluating the genotoxic effects of 8-hydroxylquinoline (8-HOQ) in loach (Misgurnus anguillicaudatus) using the micronucleus, comet and RAPD assays. In the micronucleus test and comet assay, the micronuclei rate (%) and three comet parameters (trailing rate, tail length and tail moment) in treated fish increased with increasing 8-HOQ concentration and treatment time. These results showed that exposure to 8-HOQ significantly induced genetic toxicity in loach blood cells. A subsequent RAPD assay also showed that 8-HOQ induced a genotoxic effect in loach. Among the 23 tested RAPD primers, 11 primers produced unique polymorphic band patterns and generated RAPD profile variations that displayed the band intensity, disappearance of bands and appearance of new bands of amplified DNA in the 8-HOQ-treated genomic DNA samples. In addition, the variation in RAPD profiles was time- and concentration-dependent. These results suggested that 8-HOQ is potentially harmful to fish and may be a toxic contaminant in the aquatic environment. (c) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：434 / 443
页数：10
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