Development of fingerprints for an Ayurvedic formulation Bhaskar Lavan churna, via Piperine estimation by high performance liquid chromatography

Bhaskar Lavan churna (BLC) is an Ayurvedic formulation containing Piper species (Piper longum, Piper nigrum) as main ingredients. This study was aimed to develop finger printing methods for well-known Ayurvedic formulation. Three batches of BLC were prepared in the laboratory and three different formulations were procured from Ayurvedic medicine shop. A HPLC method was developed for the estimation of Piperine in laboratory and marketed formulations. The concentration of Piperine in raw material was found to be 3.6% +/- 0.31w/w in Piper nigrum fruits, 1.4% +/- 0.27w/w in Piper longum fruits and 1.02 +/- 0.21 w/w in Piper longum roots. The content of Piperine in laboratory formulations were found to be (0.19 +/- 0.61%) and in different marketed formulations of BLC were, for BLC-A (0.10 +/- 0.21 %), BLC-B (0.092 +/- 0.34 %), BLC-C (0.071 +/- 0.52 %) w/w respectively. In order to obtain precision and accuracy the recovery study was performed and result obtained with mean value 99.36% +/- 0.25, which prove reproducibility of the result. This show significant precision of methods at 95% confidence level. The mean of % RSD value was found to be 0.35 with the mean standard error 0.242. Results of statistical analysis show present HPLC method for determination of Piperine is simple, precise, accurate and suitable for routine analysis of Piperine in BLC. The developed fingerprints can be used as a standard and Piperine can be used as a possible marker compound for fingerprinting of BLC.