Staphylococcus aureus is closely associated with food poisoning incidences, more, because of intoxication due to contamination of food with its enterotoxins. Staphylococcus enterotoxin A (SEA) is highly resistant to heat and other food processing conditions and has been implicated in food borne diseases from dairy products. Here we describe a rapid, gold nanoparticle based lateral flow immunoassay (LFIA) for detecting SEA in milk. Assay is in sandwich format and uses two specific monoclonal antibodies against SEA, one of which is labeled with gold nanoparticles and other is immobilized at control line. We also demonstrate the effect of using a combination of additives in capture antibody in order to enhance assay sensitivity. The developed LFIA is specific and has a lower detection limit of 0.5 mu g/ml. LFIA is tested both on spiked buffer and milk samples and display good reproducibility. The assembled strips stable for a month at 4 degrees C. The method may be suitable for testing SEA in milk samples as it is quick, easy to perform and reproducible. (C) 2017 Elsevier B.V. All rights reserved.
机构:
Iran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, IranIran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, Iran
Forouzani-Moghaddam, Mohamad Javad
Habibi, Sina
论文数: 0引用数: 0
h-index: 0
机构:
Iran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, Iran
Iran Univ Med Sci, Fac Allied Med, Dept Hematol & Blood Banking, Tehran, IranIran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, Iran
Habibi, Sina
Hosseini-Safa, Ahmad
论文数: 0引用数: 0
h-index: 0
机构:
Iran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, IranIran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, Iran
Hosseini-Safa, Ahmad
Khanaliha, Khadijeh
论文数: 0引用数: 0
h-index: 0
机构:
Iran Univ Med Sci, Inst Immunol & Infect Dis, Res Ctr Pediat Infect Dis, Tehran, IranIran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, Iran
Khanaliha, Khadijeh
Mokarinejad, Roya
论文数: 0引用数: 0
h-index: 0
机构:
Iran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, IranIran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, Iran
Mokarinejad, Roya
Akhoundzadeh, Fatemeh
论文数: 0引用数: 0
h-index: 0
机构:
Iran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, IranIran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, Iran
Akhoundzadeh, Fatemeh
Oshaghi, Mojgan
论文数: 0引用数: 0
h-index: 0
机构:
Iran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, IranIran Univ Med Sci, Fac Allied Med, Dept Med Lab Sci, Tehran, Iran
机构:
Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USAUniv Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA
Heidinger, Joelle C.
Winter, Carl K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USAUniv Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA
Winter, Carl K.
Cullor, James S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif, Sch Vet Med, Vet Med Teaching & Res Ctr, Dept Populat Hlth & Reprod, Tulare, CA 93274 USAUniv Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA
机构:
Key Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural UniversityKey Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University
Dang Fang-fang
Jiang Yu-jun
论文数: 0引用数: 0
h-index: 0
机构:
Key Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural UniversityKey Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University
Jiang Yu-jun
Pan Rui-li
论文数: 0引用数: 0
h-index: 0
机构:
Key Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural UniversityKey Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University
Pan Rui-li
Zhuang Ke-jin
论文数: 0引用数: 0
h-index: 0
机构:
Key Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural UniversityKey Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University
Zhuang Ke-jin
Wang Hui
论文数: 0引用数: 0
h-index: 0
机构:
Key Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural UniversityKey Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University
Wang Hui
Sun Lu-hong
论文数: 0引用数: 0
h-index: 0
机构:
Key Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural UniversityKey Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University
Sun Lu-hong
Wang Rui
论文数: 0引用数: 0
h-index: 0
机构:
Key Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural UniversityKey Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University
Wang Rui
Zhao Feng
论文数: 0引用数: 0
h-index: 0
机构:
Key Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural UniversityKey Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University
Zhao Feng
Li Tie-jing
论文数: 0引用数: 0
h-index: 0
机构:
Key Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural UniversityKey Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University
Li Tie-jing
Man Chao-xin
论文数: 0引用数: 0
h-index: 0
机构:
Key Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural UniversityKey Lab of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University
机构:
Tokyo Univ Agr & Technol, Lab Vet Publ Hlth, Fac Agr, Fuchu, Tokyo 1838509, Japan
Benha Univ, Dept Food Control, Fac Vet Med, Moshtohor 13736, Toukh, EgyptTokyo Univ Agr & Technol, Lab Vet Publ Hlth, Fac Agr, Fuchu, Tokyo 1838509, Japan
Sabike, I. I.
论文数: 引用数:
h-index:
机构:
Fujikawa, H.
Sakha, M. Z.
论文数: 0引用数: 0
h-index: 0
机构:
Tokyo Univ Agr & Technol, Lab Vet Publ Hlth, Fac Agr, Fuchu, Tokyo 1838509, JapanTokyo Univ Agr & Technol, Lab Vet Publ Hlth, Fac Agr, Fuchu, Tokyo 1838509, Japan
Sakha, M. Z.
Edris, A. M.
论文数: 0引用数: 0
h-index: 0
机构:
Benha Univ, Dept Food Control, Fac Vet Med, Moshtohor 13736, Toukh, EgyptTokyo Univ Agr & Technol, Lab Vet Publ Hlth, Fac Agr, Fuchu, Tokyo 1838509, Japan