Quantitative shotgun proteomics: considerations for a high-quality workflow in immunology

被引：69

作者：

Meissner, Felix ^{[1
]}

Mann, Matthias ^{[1
]}

机构：

[1] Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

来源：

NATURE IMMUNOLOGY | 2014年 / 15卷 / 02期

关键词：

MASS-SPECTROMETRY; CELL-CULTURE; AMINO-ACIDS; EXPRESSION; STRATEGIES; ACCURATE; REVEALS; NETWORK;

D O I：

10.1038/ni.2781

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Proteomics based on high-resolution mass spectrometry has become a powerful tool for the analysis of protein abundance, modifications and interactions. Here we describe technical aspects of proteomics workflows, instrumentation as well as computational considerations to obtain high-quality proteomics data.

引用

页码：112 / 117

页数：6

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