Quantitative shotgun proteomics: considerations for a high-quality workflow in immunology

被引：69
|
作者：
Meissner, Felix [1 ]
Mann, Matthias [1 ]
机构：
[1] Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany
来源：
NATURE IMMUNOLOGY | 2014年 / 15卷 / 02期
关键词：
MASS-SPECTROMETRY; CELL-CULTURE; AMINO-ACIDS; EXPRESSION; STRATEGIES; ACCURATE; REVEALS; NETWORK;
D O I：
10.1038/ni.2781
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Proteomics based on high-resolution mass spectrometry has become a powerful tool for the analysis of protein abundance, modifications and interactions. Here we describe technical aspects of proteomics workflows, instrumentation as well as computational considerations to obtain high-quality proteomics data.
引用
收藏
页码：112 / 117
页数：6
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