Mating for male-derived prostaglandin: a functional explanation for the increased fecundity of mated female crickets?

Direct benefits are considered to be the driving force of high female mating rates, yet species in which females do not receive material resources from males still experience increased fitness from mating frequently. One hypothesis suggests that substances within the ejaculate may boost survival or offspring production. If these materials are limiting to females, they will require continual renewal via mating and could provide a functional understanding of how high mating rates lead to increased female fitness. Using the Texas field cricket, Gryllus texensis, we investigated the sexual transfer of prostaglandin E-2, an important mediator of invertebrate reproduction. We determined that like other gryllid species, males include significant quantities of prostaglandin E-2 (PGE(2)) and its precursor molecule, arachidonic acid (AA), within the spermatophore. These components are passed to females during copulation and then stored within the spermatheca. We then tested the novel hypothesis that PGE(2) is ephemerally available after mating and that females must frequently mate to maintain access to this limiting compound. We found that PGE(2) within the spermatheca is indeed depleted through time, with only a small amount remaining 1 week after mating, but that its presence can be maintained at high quantities and for prolonged periods of time by remating. Our results support the hypothesis that high female mating rates increase the amount and availability of PGE(2) throughout the breeding season, which could explain the positive relationship between female mating rate and fecundity.