NMR assignment of the nonstructural protein nsp3(1066-1181) from SARS-CoV

被引：4
|
作者：
Serrano, Pedro [1 ]
Johnson, Margaret A. [1 ]
Chatterjee, Amarnath [1 ]
Pedrini, Bill [1 ]
Wuethrich, Kurt [1 ,2 ]
机构：
[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
来源：
BIOMOLECULAR NMR ASSIGNMENTS | 2008年 / 2卷 / 02期
关键词：
Severe acute respiratory syndrome; SARS coronavirus; Nonstructural protein; NMR structure determination;
D O I：
10.1007/s12104-008-9104-x
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Sequence-specific NMR assignments of the globular core comprising the residues 1066-1181 within the non-structural protein nsp3e from the SARS coronavirus have been obtained using triple-resonance NMR experiments with the uniformly [C-13, N-15]-labeled protein. The backbone and side chain assignments are nearly complete, providing the basis for the ongoing NMR structure determination. A preliminary identification of regular secondary structures has been derived from the C-13 chemical shifts.
引用
收藏
页码：135 / 138
页数：4
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