E4BP4 inhibits AngII-induced apoptosis in H9c2 cardiomyoblasts by activating the PI3K-Akt pathway and promoting calcium uptake

被引：13

作者：

Chen, Bih-Cheng ^{[1
]}

Shibu, Marthandam Asokan ^{[2
]}

Kuo, Chia-Hua ^{[3
]}

Shen, Chia-Yao ^{[4
]}

Chang-Lee, Shu Nu ^{[5
]}

Lai, Chao-Hung ^{[6
]}

Chen, Ray-Jade ^{[7
]}

Yao, Chun-Hsu ^{[8
]}

Viswanadha, Vijaya Padma ^{[9
]}

Liu, Jian-Shen ^{[10
,11
]}

Chen, Wei-Kung ^{[1
]}

Huang, Chih-Yang ^{[2
,12
,13
]}

机构：

[1] China Med Univ, Sch Postbaccalaureate Chinese Med, Taichung, Taiwan

[2] China Med Univ, Grad Inst Basic Med Sci, Taichung, Taiwan

[3] Univ Taipei, Lab Exercise Biochem, Taipei, Taiwan

[4] Meiho Univ, Dept Nursing, Pingtung, Taiwan

[5] Asia Univ, Dept Healthcare Adm, Taichung, Taiwan

[6] Gen Hosp, Armed Force Taiclumg, Dept Internal Med, Div Cardiol, Taichung 41152, Taiwan

[7] Taipei Med Univ, Sch Med, Dept Surg, Coll Med, Taipei, Taiwan

[8] China Med Univ, Dept Biomed Imaging & Radiol Sci, Taichung, Taiwan

[9] Bharathiar Univ, Dept Biotechnol, Coimbatore 641046, Tamil Nadu, India

[10] China Med Univ, Beigang Hosp, Dept Internal Med, Div Pulm & Crit Care Med, Beigang Township, Yunlin, Taiwan

[11] China Med Univ Hosp, Dept Emergency Med, Taichung, Taiwan

[12] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung, Taiwan

[13] China Med Univ, Grad Inst Chinese Med Sci, Taichung, Taiwan

来源：

EXPERIMENTAL CELL RESEARCH | 2018年 / 363卷 / 02期

关键词：

E4 promoter-binding protein 4; Hypertrophy; Angiotensin II; Calcium channels; INDUCED CARDIAC-HYPERTROPHY; LEFT-VENTRICULAR DYSFUNCTION; HYPERTENSIVE-RAT HEART; GROWTH-FACTOR-II; G-ALPHA-Q; ANGIOTENSIN-II; MYOCARDIAL-INFARCTION; GENE-EXPRESSION; TRANSCRIPTIONAL REPRESSION; BZIP FAMILY;

D O I：

10.1016/j.yexcr.2018.01.012

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The bZIP transcription factor E4BP4 is a survival factor that is known to be elevated in diseased heart and promote cell survival. In this study the role of E4BP4 on angiotensin-II (AngII)-induced apoptosis has been examined in in vitro cell model. H9c2 cardiomyoblast cells that overexpressed E4BP4 were exposed to AngIl to observe the cardio-protective effects of E4BP4 on hypertension related apoptosis. The results from TUNEL assays revealed that E4BP4 significantly attenuated AngII-induced apoptosis. Further analysis by Western blot and RTPCR showed that E4BP4 inhibited Angll-induced IGF-II mRNA expression and cleavage of caspase-3 through the PI3K-Akt pathway. In addition, E4BP4 enhanced calcium reuptake into the sacroplasmic reticulum by down regulating PP2A and by up-regulating the phosphorylation of PKA and PLB proteins. Our findings indicate that E4BP4 functions as a survival factor in cardiomyoblasts by inhibiting IGF-II transcription and by regulating calcium cycling.

引用

页码：227 / 234

页数：8

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