Preparation, characterization and application of organic-inorganic hybrid caffeine imprinted monolith

被引:21
|
作者
Liu, Xiaofang
Sun, Na
Zhu, Quanfei
Wu, Mei
Ye, Yong
Chen, Huaixia
机构
[1] Hubei Collaborat Innovat Ctr Adv Organ Chem Mat, Wuhan 430062, Peoples R China
[2] Key Lab Synth & Applicat Organ Funct Mol, Minist Educ, Wuhan 430062, Peoples R China
关键词
Organic-inorganic hybrid molecular; imprinting monolith; Polymer monolith microextraction; High-performance liquid chromatography; Caffeine; Children's milk; PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE MICROEXTRACTION; CAPILLARY ELECTROCHROMATOGRAPHY; BREAST-MILK; SELECTIVE RECOGNITION; PHENYLALANINE ISOMERS; EXTRACTION; SEPARATION; GEL; SPECTROMETRY;
D O I
10.1016/j.chroma.2013.06.060
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The present work aims to synthesize an organic-inorganic hybrid caffeine imprinted monolith using one-step method. The synthesis conditions such as the type of inorganic precursor and porogenic solvent, the molar ratios of the monomer and cross-linker, the volume ratio of the inorganic alcoholysate and organic part were optimized. The morphology of the monolith was studied by scanning electron microscopy and Fourier transform infrared spectra. The imprinted factor of the monolith for caffeine reached 3.02. A simple, rapid and sensitive method for the determination of caffeine in children's milk using the organic-inorganic hybrid caffeine imprinted polymer monolith microextraction combined with high-performance liquid chromatography-photodiodes array detector was developed. Several parameters affecting the sample pretreatment were investigated, including the type, flow rate and volume of eluent, the flow rate and volume of sample solution. The assay exhibited a linear dynamic range of 8-500 mu g L-1 with the correlation coefficient above 0.9987. Lower limits of detection (LOD, at S/N=3) and quantification (LOQ, at S/N=10) in children's milk samples were 2.7 and 8 mu g L-1. Recoveries of caffeine from spiked children's milk ranged from 85 to 104% with relative standard deviations of less than 8.9%. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:10 / 17
页数:8
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