Validation of a fast real-time PCR method to detect fraud and mislabeling in milk and dairy products

被引：65
|
作者：
Di Domenico, M. [1 ]
Di Giuseppe, M. [1 ]
Rodriguez, J. D. Wicochea [1 ]
Camma, C. [1 ]
机构：
[1] Ist Zooprofilat Sperimentale Abruzzo & Molise G C, Ric & Sviluppo Biotecnol, Via Campo Boario, I-64100 Teramo, Italy
来源：
JOURNAL OF DAIRY SCIENCE | 2017年 / 100卷 / 01期
关键词：
species identification; dairy products; isoelectric focusing; real-time PCR; POLYMERASE-CHAIN-REACTION; WATER-BUFFALO MOZZARELLA; BOVINE-MILK; QUANTITATIVE DETECTION; GOATS MILK; SPECIES IDENTIFICATION; COWS MILK; SHEEPS MILK; CHEESE; QUANTIFICATION;
D O I：
10.3168/jds.2016-11695
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Fast real-time PCR TaqMan assays were developed and validated for species identification in dairy products. Based on the amplification of 12S rRNA and cytB partial genes of mitochondrial DNA, the methods were demonstrated to be sensitive, fast, and species-specific for Bos taurus, Ovis aries, Bubalus bubalis, and Capra hircus. The limit of detection calculated was lower than 1%, and the efficiency was reported to be higher than 96% in every assay. An internal amplification control was used to detect possible false negatives. The method was validated by means of laboratory-prepared samples mixing different species. Moreover, 18 commercial dairy samples were analyzed by both real-time PCR and isoelectric focusing, the official European Union reference method. The 4 TaqMan assays were confirmed to be a useful tool for milk and dairy product authentication.
引用
收藏
页码：106 / 112
页数：7
相关论文
共 50 条
  • [21] Validation of real-time PCR for detection of six major pathogens in seafood products
    Taminiau, B.
    Korsak, N.
    Lemaire, C.
    Delcenserie, V.
    Daube, G.
    FOOD CONTROL, 2014, 44 : 130 - 137
  • [22] SYBR green real-time PCR method to detect Clostridium botulinum type A
    Fenicia, Lucia
    Anniballi, Fabrizio
    De Medici, Dario
    Delibato, Elisabetta
    Aureli, Paolo
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (09) : 2891 - 2896
  • [23] Development and validation of a real-time PCR detection method for celery in food
    Christine Hupfer
    Hans-Ulrich Waiblinger
    Ulrich Busch
    European Food Research and Technology, 2007, 225 : 329 - 335
  • [24] Development and validation of a real-time PCR detection method for celery in food
    Hupfer, Christine
    Waiblinger, Hans-Ulrich
    Busch, Ulrich
    EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2007, 225 (3-4) : 329 - 335
  • [25] Rapid Detection of Listeria monocytogenes by Real-Time PCR in Processed Meat and Dairy Products
    Heo, Eun Jeong
    Song, Bo Ra
    Park, Hyun Jung
    Kim, Young Jo
    Moon, Jin San
    Wee, Sung Hwan
    Kim, Jin-Seok
    Yoon, Yohan
    JOURNAL OF FOOD PROTECTION, 2014, 77 (03) : 453 - 458
  • [26] Detection and Quantification of Milk Ingredients as Hidden Allergens in Meat Products by a Novel Specific Real-Time PCR Method
    Villa, Caterina
    Costa, Joana
    Mafra, Isabel
    BIOMOLECULES, 2019, 9 (12)
  • [27] Identification of Ingredient in Mullet Roe Products by the Real-Time PCR Method
    Je-Hung Kuo
    Hsiu-Wei Tsuei
    Zih-Ling Jia
    Che-Yang Lin
    Yuan-Hsin Chang
    Bo-Lin Chen
    Jung Kuan
    Hsu-Yang Lin
    Lih-Ching Chiueh
    Daniel Yang-Chih Shih
    Hwei-Fang Cheng
    Food Analytical Methods, 2018, 11 : 992 - 1000
  • [28] Identification of Ingredient in Mullet Roe Products by the Real-Time PCR Method
    Kuo, Je-Hung
    Tsuei, Hsiu-Wei
    Jia, Zih-Ling
    Lin, Che-Yang
    Chang, Yuan-Hsin
    Chen, Bo-Lin
    Kuan, Jung
    Lin, Hsu-Yang
    Chiueh, Lih-Ching
    Shih, Daniel Yang-Chih
    Cheng, Hwei-Fang
    FOOD ANALYTICAL METHODS, 2018, 11 (04) : 992 - 1000
  • [29] Development and validation of a multiplex real-time PCR method to simultaneously detect 47 targets for the identification of genetically modified organisms
    Cottenet, Geoffrey
    Blancpain, Carine
    Sonnard, Veronique
    Chuah, Poh Fong
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2013, 405 (21) : 6831 - 6844
  • [30] Development and validation of a multiplex real-time PCR method to simultaneously detect 47 targets for the identification of genetically modified organisms
    Geoffrey Cottenet
    Carine Blancpain
    Véronique Sonnard
    Poh Fong Chuah
    Analytical and Bioanalytical Chemistry, 2013, 405 : 6831 - 6844
12345