Isolation and characterization of polymorphic microsatellite loci from the endangered Tarim schizothoracin (Schizothorax biddulphi Gunther)

被引:3
|
作者
Gong, Xiaoling [2 ]
Cui, Zhongkai [2 ]
Wang, Chenghui [1 ]
机构
[1] Shanghai Ocean Univ, Key Lab Freshwater Aquat Genet Resources, Minist Agr, Shanghai 201306, Peoples R China
[2] Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China
关键词
Schizothorax biddulphi; Microsatellite; Genetic variation;
D O I
10.1007/s12686-012-9646-1
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Tarim schizothoracin (Schizothorax biddulphi Gunther) is an extremely endangered freshwater fish in China. In this paper, 15 polymorphic microsatellite loci were characterized in this species. The number of alleles ranged from 4 to 14, the observed heterozygosities (H (O)) were from 0.7609 to 1.0000, and the expected heterozygosities (H (E)) ranged from 0.7273 to 0.9194. The polymorphism information content was from 0.6684 to 0.9020. Only two loci deviated significantly from the Hardy-Weinberg equilibrium, and none of the loci combinations showed significant linkage disequilibrium. These microsatellite loci are useful in investigating the genetic variability in Tarim schizothoracin.
引用
收藏
页码:795 / 797
页数:3
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