Escherichia coli-based cell-free extract development for protein-based cancer therapeutic production

被引：7
|
作者：
Salehi, Amin S. M. [1 ]
Earl, Conner C. [1 ]
Muhlestein, Christina [1 ]
Bundy, Bradley C. [1 ]
机构：
[1] Brigham Young Univ, Dept Chem Engn, 350S Clyde Bldg, Provo, UT 84602 USA
来源：
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY | 2016年 / 60卷 / 7-9期
基金：
美国国家科学基金会;
关键词：
cell-free protein synthesis; cell extract; cancer research; anticancer biotherapeutics; SITE-SPECIFIC INCORPORATION; AMINO-ACID STABILIZATION; FREE EXPRESSION; RAPID EXPRESSION; FREE TRANSLATION; FREE SYSTEM; PEPTIDES; ONCONASE; ROBUST; STABILITY;
D O I：
10.1387/ijdb.160125bb
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Cell-free protein synthesis has been around for decades but it has never been close to becoming a robust tool for the production of biotherapeutic agents. In this review, we focus on how Escherichia coli-based cell-free protein synthesis can be modified in various ways to produce challenging, complex anticancer biotherapeutics. Here we report progress in extract preparation and its relation to cell-free cancer research. The future prospects of cell-free technology and its potential in various areas of cancer therapeutics production are also highlighted.
引用
收藏
页码：237 / 243
页数：7
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