Sexing European rabbits (Oryctolagus cuniculus), European brown hares (Lepus europaeus) and mountain hares (Lepus timidus) with ZFX and ZFY loci

被引:12
|
作者
Fontanesi, Luca [1 ]
Tazzoli, Marco [1 ]
Pecchioli, Elena [2 ]
Hauffe, Heidi C. [2 ]
Robinson, Terence J. [3 ]
Russo, Vincenzo [1 ]
机构
[1] Univ Bologna, Fac Agr, Sez Allevamenti Zootecnici, DIPROVAL, I-42100 Reggio Emilia, Italy
[2] Ctr Ecol Alpina, I-38040 Trento, Italy
[3] Univ Stellenbosch, Evolutionary Genom Grp, Dept Bot & Zool, ZA-7602 Matieland, South Africa
关键词
hare; Leporidae; molecular sexing; rabbit; ZFX; ZFY;
D O I
10.1111/j.1755-0998.2008.02167.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We describe a new method for the sex determination of tissue originating from Oryctolagus cuniculus (European rabbit), Lepus europaeus (European brown hare) and Lepus timidus (mountain hare) based on PCR-RFLP analysis of point mutations that differentiate the ZFX and ZFY gene sequences. Among several applications, this PCR-RFLP method could be used to investigate gender ratio and evaluate the population dynamics of these species using samples collected when sex cannot be identified.
引用
收藏
页码:1294 / 1296
页数:3
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