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Sexing European rabbits (Oryctolagus cuniculus), European brown hares (Lepus europaeus) and mountain hares (Lepus timidus) with ZFX and ZFY loci
被引:12
|作者:
Fontanesi, Luca
[1
]
Tazzoli, Marco
[1
]
Pecchioli, Elena
[2
]
Hauffe, Heidi C.
[2
]
Robinson, Terence J.
[3
]
Russo, Vincenzo
[1
]
机构:
[1] Univ Bologna, Fac Agr, Sez Allevamenti Zootecnici, DIPROVAL, I-42100 Reggio Emilia, Italy
[2] Ctr Ecol Alpina, I-38040 Trento, Italy
[3] Univ Stellenbosch, Evolutionary Genom Grp, Dept Bot & Zool, ZA-7602 Matieland, South Africa
关键词:
hare;
Leporidae;
molecular sexing;
rabbit;
ZFX;
ZFY;
D O I:
10.1111/j.1755-0998.2008.02167.x
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
We describe a new method for the sex determination of tissue originating from Oryctolagus cuniculus (European rabbit), Lepus europaeus (European brown hare) and Lepus timidus (mountain hare) based on PCR-RFLP analysis of point mutations that differentiate the ZFX and ZFY gene sequences. Among several applications, this PCR-RFLP method could be used to investigate gender ratio and evaluate the population dynamics of these species using samples collected when sex cannot be identified.
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页码:1294 / 1296
页数:3
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