We describe a new method for the sex determination of tissue originating from Oryctolagus cuniculus (European rabbit), Lepus europaeus (European brown hare) and Lepus timidus (mountain hare) based on PCR-RFLP analysis of point mutations that differentiate the ZFX and ZFY gene sequences. Among several applications, this PCR-RFLP method could be used to investigate gender ratio and evaluate the population dynamics of these species using samples collected when sex cannot be identified.
机构:
Univ Vet Med Vienna, Res Inst Wildlife Ecol, Savoyenstr 1, A-1160 Vienna, AustriaUniv Vet Med Vienna, Res Inst Wildlife Ecol, Savoyenstr 1, A-1160 Vienna, Austria
Posautz, Annika
Kuebber-Heiss, Anna
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机构:
Univ Vet Med Vienna, Res Inst Wildlife Ecol, Savoyenstr 1, A-1160 Vienna, AustriaUniv Vet Med Vienna, Res Inst Wildlife Ecol, Savoyenstr 1, A-1160 Vienna, Austria
Kuebber-Heiss, Anna
Loncaric, Igor
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Univ Vet Med Vienna, Inst Microbiol, Vet Pl 1, A-1210 Vienna, AustriaUniv Vet Med Vienna, Res Inst Wildlife Ecol, Savoyenstr 1, A-1160 Vienna, Austria
Loncaric, Igor
5TH INTERNATIONAL CONFERENCE - AGRICULTURE FOR LIFE, LIFE FOR AGRICULTURE,
2016,
10
: 358
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363