Cloning and expression of an endo-1,3-1,4-beta-glucanase gene from Bacillus macerans in Lactobacillus reuteri

被引：24
|
作者：
Heng, NCK
Jenkinson, HF
Tannock, GW
机构：
[1] UNIV OTAGO,DEPT MICROBIOL,DUNEDIN,NEW ZEALAND
[2] UNIV OTAGO,DEPT ORAL BIOL,DUNEDIN,NEW ZEALAND
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 08期
关键词：
D O I：
10.1128/AEM.63.8.3336-3340.1997
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Strains of the gastrointestinal species Lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3-1,4-beta-glucanase gene (bglM) of Bacillus macerans. The enzyme was expressed and secreted by the lactobacilli. A plasmid construct containing the bglM gene lacking its promoter was derived and was demonstrated to be useful as a promoter probe vector.
引用
收藏
页码：3336 / 3340
页数：5
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