Antibacterial activity of polyeugenol against staphylococcus aureus and escherichia coli

Antibacterial activity of polypugenol compounds has been carried out in the form active ingredients and gels against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). Antibacterial activity assay of polyeugenol in active form with varied concentration 0.0025 g/mL, 0.005 g/mL, 0.01 g/mL, 0.02 g/mL, and 0.04 g/mL and gelling form varied concentration 0.01 g/mL, 0.02 g/mL, and 0.04 g/mL. Antibacterial activity assay using agar disk diffusion method by measuring the diameter of inhibition.The result showed that antibacterial activity not presence at Escherichia coli while Staphylococcus aureus has presence of antibacterial activity at concentration 0.01 g/mL, 0.02 g/mL, dan 0.04 g/mL with inhibition diameter values 15.72; 17.28 and 20.51 mm. Polyeugenol in gelling form were tested against Staphylococcus aureus and Escherichia coli at concentration 0.01g/mL, 0.02g/mL, dan 0.04g/mL. The presence of antibacterial activity obtained for Escherichia coli only at concentration 0.04 g/mL with the value of inhibition diameter is 0.53 mm while concentration 0.01g/mL dan 0.02g/mL not presence antibacterial activity. The presence of antibacterial activity for Staphylococcus aureus obtained at concentration 0.01 g/mL, 0.02 g/mL and 0.04 g/mL with the value of inhibition diameter are 14.46; 15.68 and 18.81 mm that showed relatively strong antibacterial activity. Statistic tested using one way ANOVA in Staphylococcus aureus shows significant differences at each concentration from polyeugenol in active form and gelling form with F-count> F-tab.