Serodiagnosis of human neurocysticercosis using antigenic components of Taenia solium metacestodes derived from the unbound fraction from jacalin affinity chromatography

被引:3
|
作者
Machado, Gleyce Alves [1 ,2 ]
de Oliveira, Heliana Batista [1 ,2 ]
Gennari-Cardoso, Margareth Leitao [1 ,3 ]
Mineo, Jose Roberto [1 ]
Costa-Cruz, Julia Maria [1 ]
机构
[1] Univ Fed Uberlandia, Inst Ciencias Biomed, Dept Imunol Microbiol & Parasitol, BR-38400 Uberlandia, MG, Brazil
[2] Univ Fed Goias, Dept Ciencias Biol, Catalao, Go, Brazil
[3] Univ Estadual Santa Cruz, Dept Ciencias Biol, Ilheus, BA, Brazil
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2013年 / 108卷 / 03期
关键词
neurocysticercosis; Taenia solium; jacalin; Triton X-114; diagnosis; CEREBROSPINAL-FLUID; IGG ANTIBODIES; DIAGNOSIS; CYSTICERCOSIS; CRASSICEPS; ARTOCARPUS; PEPTIDES; PROTEINS; SAMPLES; IMMUNODIAGNOSIS;
D O I
10.1590/S0074-02762013000300016
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The aim of the present study was to analyse Taenia solium metacestode antigens that were derived from the unbound fraction of jacalin affinity chromatography and subsequent tert-octylphenoxy poly(oxyethylene) ethanol Triton X-114 (TX-114) partitioning in the diagnosis of human neurocysticercosis (NCC). Immunoassays were designed to detect T. solium-specific IgG antibodies by ELISA and immunoblot. Serum samples were collected from 132 individuals who were categorised as follows: 40 had NCC, 62 presented Taenia spp or other parasitic diseases and 30 were healthy individuals. The jacalin-unbound (J(unbound)) fraction presented higher sensitivity and specificity rates than the jacalin-bound fraction and only this fraction was subjected to subsequent TX-114 partitioning, resulting in detergent (DJ(unbound)) and aqueous (AJ(unbound)) fractions. The ELISA sensitivity and specificity were 85% and 84.8% for J(unbound), 92.5% and 93.5% for DJ(unbound) and 82.5% and 82.6% for AJ(unbound). By immunoblot, the DJ(unbound) fraction showed 100% sensitivity and specificity and only serum samples from patients with NCC recognised the 50-70 kDa T. solium-specific components. We conclude that the DJ(unbound) fraction can serve as a useful tool for the differential immunodiagnosis of NCC by immunoblot.
引用
收藏
页码:368 / 375
页数:8
相关论文
共 50 条
  • [31] STUDIES ON PLASMINOGEN .9. PURIFICATION OF HUMAN PLASMINOGEN FROM COHN FRACTION-III BY AFFINITY CHROMATOGRAPHY
    LIU, TH
    MERTZ, ET
    CANADIAN JOURNAL OF BIOCHEMISTRY, 1971, 49 (09): : 1055 - +
  • [32] Purification of human butyrylcholinesterase from frozen Cohn fraction IV-4 by ion exchange and Hupresin affinity chromatography
    Schopfer, Lawrence M.
    Lockridge, Oksana
    David, Emilie
    Hinrichs, Steven H.
    PLOS ONE, 2019, 14 (01):
  • [33] Affinity chromatography of porcine pepsin and pepsinogen using immobilized ligands derived from the specific substrate for this enzyme
    Frydlová, J
    Kucerová, Z
    Tichá, M
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 800 (1-2): : 109 - 114
  • [34] Enrichment of Amadori products derived from the nonenzymatic glycation of proteins using microscale boronate affinity chromatography
    Takatsy, Aniko
    Boddi, Katalin
    Nagy, Livia
    Nagy, Geza
    Szabo, Szilvia
    Marko, Lajos
    Wittmann, Istvan
    Ohmacht, Robert
    Ringer, Thomas
    Bonn, Guenther K.
    Gjerde, Douglas
    Szabo, Zoltan
    ANALYTICAL BIOCHEMISTRY, 2009, 393 (01) : 8 - 22
  • [35] PURIFICATION OF LIPOPROTEIN-LIPASE FROM HUMAN ADIPOSE-TISSUE USING AFFINITY CHROMATOGRAPHY
    ETIENNE, J
    BRETON, M
    VANHOVE, A
    POLONOVSKI, J
    COMPTES RENDUS HEBDOMADAIRES DES SEANCES DE L ACADEMIE DES SCIENCES SERIE D, 1974, 279 (17): : 1487 - 1490
  • [36] A RAPID PROCEDURE FOR THE PURIFICATION OF IGA1 AND IGA2 SUBCLASSES FROM NORMAL HUMAN-SERUM USING PROTEIN-G AND JACKFRUIT LECTIN (JACALIN) AFFINITY-CHROMATOGRAPHY
    HAUN, M
    INCLEDON, B
    ALLES, P
    WASI, S
    IMMUNOLOGY LETTERS, 1989, 22 (04) : 273 - 280
  • [37] Partially purified fraction (PPF) antigen from adult Fasciola gigantica for the serodiagnosis of human fascioliasis using Dot-ELISA technique
    Dalimi, A
    Hadighi, R
    Madani, R
    ANNALS OF SAUDI MEDICINE, 2004, 24 (01) : 18 - 20
  • [38] PURIFICATION OF HUMAN FACTOR-XII FROM PLASMA USING ZINC CHELATE AFFINITY-CHROMATOGRAPHY
    PIXLEY, RA
    COLMAN, RW
    THROMBOSIS RESEARCH, 1986, 41 (01) : 89 - 98
  • [39] PURIFICATION OF GLUTATHIONE-S-TRANSFERASES FROM HUMAN LIVER USING GLUTATHIONE-AFFINITY CHROMATOGRAPHY
    SIMONS, PC
    FEDERATION PROCEEDINGS, 1977, 36 (03) : 760 - 760
  • [40] Purification of human prothrombin from Nitschmann fraction III using DEAE membrane radial flow chromatography
    Sun, T
    Chen, G
    Liu, YP
    Bu, FR
    Wen, MJ
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2000, 742 (01): : 109 - 114