House dust mite allergen Der f 1 induces IL-8 in human basophilic cells via ROS-ERK and p38 signal pathways

被引:11
|
作者
Yi, Myung-hee [1 ,2 ,3 ]
Kim, Hyoung-Pyo [1 ,2 ]
Jeong, Kyoung Yong [4 ]
Kim, Chung-Ryul [1 ,2 ,5 ]
Kim, Tae Yun [1 ,2 ]
Yong, Tai-Soon [1 ,2 ]
机构
[1] Yonsei Univ, Coll Med, Inst Trop Med, Dept Environm Med Biol, Seoul 120752, South Korea
[2] Yonsei Univ, Coll Med, Inst Trop Med, Arthropods Med Importance Resource Bank, Seoul 120752, South Korea
[3] Yonsei Univ, Brain Korea PLUS Project Med Sci 21, Seoul 120749, South Korea
[4] Yonsei Univ, Coll Med, Inst Allergy, Dept Internal Med, Seoul 120752, South Korea
[5] Ajou Univ, Sch Med, Dept Microbiol, Suwon 443721, South Korea
基金
新加坡国家研究基金会;
关键词
Basophil; KU812; Cysteine protease; Der f 1; IL-8; INTERLEUKIN-8; GENE-EXPRESSION; RESPONSES; DER-P-1; IGE; HYPERSENSITIVITY; PHOSPHORYLATION; EOSINOPHILS; RECRUITMENT; ACTIVATION; SECRETION;
D O I
10.1016/j.cyto.2015.07.011
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Der f 1, a major house dust mite allergen and member of the papain-like cysteine protease family, can provoke immune responses with its proteolytic activity. To understand the role of Der f 1 in inflammatory immune responses, we studied the mechanism of the regulation of interleukin (IL)-8 expressions in human basophilic cell KU812 by proteolytically active recombinant Der f 1. Not only production of IL-8 mRNA was induced but also the DNA binding activity of activator protein-1 (AP-1) and phosphorylation of NF-kappa B p65 were increased in Der f 1-treated KU812. Furthermore, Der f I induction of IL-8 expression was sensitive to pharmacological inhibition of ERK and p38 mitogen activated protein kinase (MAPK) pathways. Der f 1 also activated ERK and p38 MAPK phosphorylation and rapidly induced reactive oxygen species (ROS) production. The antioxidant N-acetyl-cysteine (NAC) inhibited phosphorylation of ERIC, but not p38, suggesting that secretion of IL-8 in KU812 cells treated with Der f 1 is dependent on ROS, ERIC MAPK and p38 MAPK. We describe the mechanism of Der f 1-induced IL-8 secretion from human basophilic cells, which are thought to be important for allergic inflammation independent of IgE antibodies. These findings improve our understanding of the inflammatory immune response in human basophils to protease allergens. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码:356 / 364
页数:9
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