Cucumber mosaic virus from Ixora: Infectious RNA transcripts confirm deficiency in satellite support and unusual symptomatology

被引：7
|
作者：
Tousignant, ME
McGarvey, PM
Piazzolla, P
Kaper, JM
机构：
[1] THOMAS JEFFERSON UNIV, INST BIOTECHNOL & ADV MOL MED, PHILADELPHIA, PA 19107 USA
[2] UNIV BASILICATA, DIPARTIMENTO BIOL DIFESA & BIOTECNOL AGRO FORESTA, I-85100 POTENZA, ITALY
来源：
RESEARCH IN VIROLOGY | 1996年 / 147卷 / 06期
关键词：
CMV; RNA; transcription; Ixora strain; RT-PCR; infectious transcripts; satellite support and replication; symptomatology;
D O I：
10.1016/S0923-2516(97)85128-X
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A cucumber mosaic virus isolate from an Ixora plant originating in the Philippines and first described 20 years ago (CMV-Ix) by Waterworth and Povish differs from other characterized CMV strains in its anomalous satellite support and in several biological and molecular properties. We describe the preparation of infectious transcripts from cloned complementary DNA and the characterization of progeny virus. The results confirm that CMV-Ix has a more limited host range than most CMV isolates. Virions of CMV-Ix are smaller than those of the control CMV-S, independent of the type of negative stain used. Furthermore, CMV-Ix from transcripts supports the replication of the satellite T-CARNA 5 but not of D-CARNA 5.
引用
收藏
页码：363 / 373
页数：11
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