Use of a Single-Layer Density Centrifugation Method Enhances Sperm Quality in Cryopreserved-Thawed Equine Spermatozoa

被引：9
|
作者：
Stoll, Anja [1 ]
Love, Charles C. [2 ]
Ball, Barry A. [1 ]
机构：
[1] Univ Calif Davis, Dept Populat Hlth & Reprod, Davis, CA 95616 USA
[2] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Large Anim Clin Sci, College Stn, TX USA
来源：
JOURNAL OF EQUINE VETERINARY SCIENCE | 2013年 / 33卷 / 07期
关键词：
Equine; Density centrifugation; Sperm quality; Sperm chromatin; Cryopreservation; GRADIENT CENTRIFUGATION; STALLION SPERMATOZOA; TYROSINE PHOSPHORYLATION; MITOCHONDRIAL-FUNCTION; SUBFERTILE STALLION; DNA FRAGMENTATION; SEMEN; MOTILITY; INTEGRITY; FILTRATION;
D O I：
10.1016/j.jevs.2012.08.009
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The objective of this study was to improve the quality of cryopreserved-thawed equine sperm using single-layer density centrifugation (SLC). Sperm quality was assessed by DNA integrity, motility, morphology, mitochondrial membrane potential, viability, and plasma membrane alteration. The percentage of DNA-damaged sperm (expressed in % COMP) was lower (P = .001) after SLC (1.6 +/- 0.5% vs. 6.8 +/- 0.5%). Total sperm motility (80 +/- 2.4% vs. 41.7 +/- 2.4%) and progressive sperm motility (69.5 +/- 2.9% vs. 31.5 +/- 2.9%) (P < .001), as well as the percentage of morphologically normal sperm (45 +/- 3.9% vs. 27.7 +/- 3.9%), increased after SLC compared with control sample. In addition, the proportion of sperm with high mitochondrial membrane potential increased (81.6 +/- 1.8% vs. 42.1 +/- 1.8%), as did the viability of sperm (71.1 +/- 2.4% vs. 39.5 +/- 2.4%), after SLC compared with the control sperm. The proportion of sperm with alteration in plasma membrane structure was lower after SLC compared with control sample (6.4 +/- 1.1% vs. 18.9 +/- 1.1%). Overall, sperm recovery was 72.+/- 7 3.6% in the control sample compared with 14.8 +/- 3.6% after SLC (P < .001). We conclude that based on the sperm parameters evaluated, SLC improves the quality of cryopreserved-thawed equine spermatozoa. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码：547 / 551
页数：5
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