Binary monolayers of single-stranded oligonucleotides and blocking agent for hybridisation

被引：10
|
作者：
Vikholm-Lundin, Inger [1 ]
Piskonen, Reetta [1 ]
机构：
[1] VTT Tech Res Ctr Finland, FI-33101 Tampere, Finland
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2008年 / 134卷 / 01期
关键词：
SH-ssDNA; Oligonucleotides; Non-specific binding; Surface plasmon resonance; Hybridisation;
D O I：
10.1016/j.snb.2008.04.027
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The hybridisation of thiol-modified single-stranded DNA (SH-ssDNA) embedded in lipoate or hydrophilic polymer monolayers on gold have been studied through the technique of surface plasmon resonance (SPR). SH-ssDNA and the blocking agents were co-adsorbed on gold from the same solution or the surface was post-treated with the blocking agents. The assembling process is allowed to take place for only 10 min. Binding of non-complementary and complementary DNA is dependent both on the blocking agent and on the assembling method used. The lowest non-complementary binding and the highest complementary binding of DNA was obtained with a layer assembled from a binary solution of SH-ssDNA and the lipoate blocking agent. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：189 / 192
页数：4
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