A turn-on upconversion fluorescence resonance energy transfer biosensor for ultrasensitive endonuclease detection

被引：10
|
作者：
Huang, Li-Jiao [1 ]
Tian, Xue [1 ]
Yi, Jin-Tao [1 ]
Yu, Ru-Qin [1 ]
Chu, Xia [1 ]
机构：
[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China
来源：
ANALYTICAL METHODS | 2015年 / 7卷 / 18期
基金：
中国国家自然科学基金;
关键词：
SENSITIVE DETECTION; NANOPARTICLES; SENSOR; NANOCRYSTALS;
D O I：
10.1039/c5ay01169h
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A facile one-step approach was proposed to make hydrophilic and DNA-functionalizable upconversion nanoparticles through ligand exchange at the liquid-liquid interface, and an ultrasensitive and selective biosensor for nuclease assay and its inhibitors assay based on FRET from the DNA-functionalizable UCNPs to graphene oxide was designed. A high sensitivity exhibited with a detectable minimum concentration of 1 x 10(-4) units per mL S1 nuclease, which was more sensitive than the developed approaches.
引用
收藏
页码：7474 / 7479
页数：6
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