A mixed hemadsorption assay for detection of cell surface binding anti-tumor antibodies in human sera

被引：2
|
作者：
Akis, N [1 ]
Andl, C [1 ]
Zhou, DW [1 ]
机构：
[1] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 2002年 / 261卷 / 1-2期
关键词：
mixed hemadsorption assay; GA733; humoral response in cancer;
D O I：
10.1016/S0022-1759(01)00560-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A practical mixed hemadsorption assay (MHA) was developed for the detection of cell surface binding anti-tumor antigen antibodies (anti-TA Ab) in the human sera. The assay was compared to enzyme-linked immunosorbent assay (ELISA) and dot ELISA in the detection of the antibodies developed against GA733-2 antigen. SW1116 cell line and recombinant GA733-2E protein were used as targets in the MHA and solid phase immunoassays (SPIA), respectively. Sera were obtained from healthy donors and vaccinated-colon carcinoma patients. The sensitivity level of the MHA was very high for detection of anti-TA Ab in sera. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：119 / 127
页数：9
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