Glucose Promotes Stress Resistance in the Fungal Pathogen Candida albicans

被引:145
|
作者
Rodaki, Alexandra [1 ]
Bohovych, Iryna M. [1 ]
Enjalbert, Brice [1 ]
Young, Tim [2 ]
Odds, Frank C. [1 ]
Gow, Neil A. R. [1 ]
Brown, Alistair J. P. [1 ]
机构
[1] Univ Aberdeen, Inst Med Sci, Aberdeen Fungal Grp, Aberdeen AB25 2ZD, Scotland
[2] Pfizer Ltd, Discovery Biol, Sandwich CT13 9NJ, Kent, England
基金
英国生物技术与生命科学研究理事会; 英国惠康基金;
关键词
ACTIVATED PROTEIN-KINASE; SACCHAROMYCES-CEREVISIAE; TRANSCRIPTIONAL RESPONSE; MULTIDRUG-RESISTANCE; DECREASES INFECTIVITY; NUCLEAR-LOCALIZATION; SENSING MECHANISMS; PLASMA-MEMBRANE; GENE-EXPRESSION; MESSENGER-RNAS;
D O I
10.1091/mbc.E09-01-0002
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Metabolic adaptation, and in particular the modulation of carbon assimilatory pathways during disease progression, is thought to contribute to the pathogenicity of Candida albicans. Therefore, we have examined the global impact of glucose upon the C. albicans transcriptome, testing the sensitivity of this pathogen to wide-ranging glucose levels (0.01, 0.1, and 1.0%). We show that, like Saccharomyces cerevisiae, C. albicans is exquisitely sensitive to glucose, regulating central metabolic genes even in response to 0.01% glucose. This indicates that glucose concentrations in the bloodstream (approximate range 0.05-0.1%) have a significant impact upon C. albicans gene regulation. However, in contrast to S. cerevisiae where glucose down-regulates stress responses, some stress genes were induced by glucose in C. albicans. This was reflected in elevated resistance to oxidative and cationic stresses and resistance to an azole antifungal agent. Cap1 and Hog1 probably mediate glucose-enhanced resistance to oxidative stress, but neither is essential for this effect. However, Hog1 is phosphorylated in response to glucose and is essential for glucose-enhanced resistance to cationic stress. The data suggest that, upon entering the bloodstream, C. albicans cells respond to glucose increasing their resistance to the oxidative and cationic stresses central to the armory of immunoprotective phagocytic cells.
引用
收藏
页码:4845 / 4855
页数:11
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