A Fast Microfluidic Temperature Control Device for Studying Microtubule Dynamics in Fission Yeast

被引：12

作者：

Velve-Casquillas, Guilhem ^{[1
]}

Costa, Judite ^{[2
]}

Carlier-Grynkorn, Frederique ^{[1
]}

Mayeux, Adeline ^{[1
]}

Tran, Phong T. ^{[1
,2
]}

机构：

[1] CNRS, Inst Curie, UMR 144, F-75005 Paris, France

[2] Univ Penn, Philadelphia, PA 19104 USA

来源：

MICROTUBULES: IN VIVO | 2010年 / 97卷

关键词：

POLY(DIMETHYLSILOXANE);

D O I：

10.1016/S0091-679X(10)97011-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recent development in soft lithography and microfluidics enables biologists to create tools to control the cellular microenvironment. One such control is the ability to quickly change the temperature of the cells. Genetic model organism such as fission yeast has been useful for studies of the cell cytoskeleton. In particular, the dynamic microtubule cytoskeleton responds to changes in temperature. In addition, there are temperature-sensitive mutations of cytoskeletal proteins. We describe here the fabrication and use of a microfluidic device to quickly and reversibly change cellular temperature between 2 degrees C and 50 degrees C. We demonstrate the use of this device while imaging at high-resolution microtubule dynamics in fission yeast.

引用

页码：185 / 201

页数：17

共 44 条

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[42] The V260I mutation in fission yeast α-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint
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[44] Lanthanide Paramagnetic Probes for NMR Spectroscopic Studies of Fast Molecular Conformational Dynamics and Temperature Control. Effective Six-Site Proton Exchange in 18-Crown-6 by Exchange Spectroscopy
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