Subunit D of the vacuolar H+-ATPase of Arabidopsis thaliana

被引：23
|
作者：
Kluge, C [1 ]
Golldack, D [1 ]
Dietz, KJ [1 ]
机构：
[1] Univ Bielefeld, Fac Biol W5, Dept Physiol & Biochem Plants, D-33501 Bielefeld, Germany
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1999年 / 1419卷 / 01期
关键词：
Arabidopsis thaliana; ATPase; cDNA; in situ hybridization; leaf; subunit D; root; vacuole;
D O I：
10.1016/S0005-2736(99)00055-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A 1034 bp cDNA encoding the full length sequence of subunit D of the vacuolar H+-ATPase was cloned from Arabidopsis thaliana. The open reading frame of the cDNA clone vatpD contains 780 bp and codes for a protein of 29.1 kDa with a pi of 9.52. Structural predictions show similarities to subunit gamma of the F-ATP synthases. Identity between subunit D of the vacuolar H+-ATPase of.4. thaliana and subunits D from other eukaryotic organisms is in the range of 57% (Bos taurus) to 48% (Candida albicans). Hybridization of genomic DNA with vatpD indicates the existence of one gene copy of subunit D in A. thaliana. Northern blot hybridization and in situ hybridization showed expression of vatpD in all cell types. The expression of subunit D was not modified by salt stress or abscisic acid treatment in A. thaliana. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：105 / 110
页数：6
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