A sensitive and specific method for microRNA detection andin situimaging based on a CRISPR-Cas9 modified catalytic hairpin assembly

被引：25
|
作者：
Liu, Yang [1 ]
Li, Shihong [1 ]
Zhang, Likun [1 ]
Zhao, Qian [1 ]
Li, Nuo [1 ]
Wu, Yuxin [1 ]
机构：
[1] Jinzhou Med Univ, Dept Clin Lab, Affiliated Hosp 3, Jinzhou 121000, Liaoning, Peoples R China
来源：
RSC ADVANCES | 2020年 / 10卷 / 47期
关键词：
MIRNA;
D O I：
10.1039/d0ra03603j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR-Cas9 and catalytic hairpin assembly. With the cascade signal amplification of catalytic hairpin assembly and high specific recognition of CRISPR-Cas9, the detection method showed a sensitivity of 23.5 fM and was applied for accurate exosomal miR-21 detection andin situmiR-21 imaging.
引用
收藏
页码：28037 / 28040
页数：4
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