A sensitive and specific method for microRNA detection andin situimaging based on a CRISPR-Cas9 modified catalytic hairpin assembly

被引：25
|
作者：
Liu, Yang [1 ]
Li, Shihong [1 ]
Zhang, Likun [1 ]
Zhao, Qian [1 ]
Li, Nuo [1 ]
Wu, Yuxin [1 ]
机构：
[1] Jinzhou Med Univ, Dept Clin Lab, Affiliated Hosp 3, Jinzhou 121000, Liaoning, Peoples R China
来源：
RSC ADVANCES | 2020年 / 10卷 / 47期
关键词：
MIRNA;
D O I：
10.1039/d0ra03603j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR-Cas9 and catalytic hairpin assembly. With the cascade signal amplification of catalytic hairpin assembly and high specific recognition of CRISPR-Cas9, the detection method showed a sensitivity of 23.5 fM and was applied for accurate exosomal miR-21 detection andin situmiR-21 imaging.
引用
收藏
页码：28037 / 28040
页数：4
相关论文
共 50 条
  • [1] A sensitive electrochemical method for rapid detection of dengue virus by CRISPR/Cas13a-assisted catalytic hairpin assembly
    Wang, Jiaojiao
    Xia, Qianfeng
    Wu, Jie
    Lin, Yingzi
    Ju, Huangxian
    ANALYTICA CHIMICA ACTA, 2021, 1187
  • [2] A simple electrochemical biosensor for highly sensitive and specific detection of microRNA based on mismatched catalytic hairpin assembly
    Zhang, Ye
    Yan, Yurong
    Chen, Wenhong
    Cheng, Wei
    Li, Shengqiang
    Ding, Xiaojuan
    Li, Dandan
    Wang, Hong
    Ju, Huangxian
    Ding, Shijia
    BIOSENSORS & BIOELECTRONICS, 2015, 68 : 343 - 349
  • [3] Sensitive detection of intracellular microRNA based on a flowerlike vector with catalytic hairpin assembly
    Liu, Jintong
    Du, Ping
    Zhang, Jing
    Shen, Hong
    Lei, Jianping
    CHEMICAL COMMUNICATIONS, 2018, 54 (20) : 2550 - 2553
  • [4] Catalytic hairpin assembly gel assay for multiple and sensitive microRNA detection
    Dai, Wenhao
    Zhang, Jing
    Meng, Xiangdan
    He, Jie
    Zhang, Kai
    Cao, Yu
    Wang, Dongdong
    Dong, Haifeng
    Zhang, Xueji
    THERANOSTICS, 2018, 8 (10): : 2646 - 2656
  • [5] Highly Effective and Low-Cost MicroRNA Detection with CRISPR-Cas9
    Qiu, Xin-Yuan
    Zhu, Lv-Yun
    Zhu, Chu-Shu
    Ma, Jia-Xin
    Hou, Tao
    Wu, Xiao-Min
    Xie, Si-Si
    Min, Lu
    Tan, De-An
    Zhang, Dong-Yi
    Zhu, Lingyun
    ACS SYNTHETIC BIOLOGY, 2018, 7 (03): : 807 - 813
  • [6] Catalytic hairpin assembly-coupled CRISPR/Cas12a biosensor for sensitive detection of melamine in dairy products
    Cheng, Hui-Juan
    Liu, Yue
    Li, Hua-Dong
    Yin, Bin-Cheng
    Ma, Pei-Qiang
    Ye, Bang-Ce
    CHEMICAL COMMUNICATIONS, 2025,
  • [7] An isothermal electrochemical biosensor for the sensitive detection of microRNA based on a catalytic hairpin assembly and supersandwich amplification
    Zhang, Hua
    Wang, Qing
    Yang, Xiaohai
    Wang, Kemin
    Li, Qing
    Li, Zhiping
    Gao, Lei
    Nie, Wenyan
    Zheng, Yan
    ANALYST, 2017, 142 (02) : 389 - 396
  • [8] Ratiometric SERS biosensor for sensitive and reproducible detection of microRNA based on mismatched catalytic hairpin assembly
    Chen, Jinyang
    Wu, Yan
    Fu, Cuicui
    Cao, Haiyan
    Tan, Xiaoping
    Shi, Wenbing
    Wu, Zhaoyang
    BIOSENSORS & BIOELECTRONICS, 2019, 143
  • [9] Ultrasensitive electrochemical assay for microRNA-21 based on CRISPR/Cas13a-assisted catalytic hairpin assembly
    Cui, Ying
    Fan, Shanji
    Yuan, Ze
    Song, Minghui
    Hu, Jiawen
    Qian, Dong
    Zhen, Deshuai
    Li, Junhua
    Zhu, Baode
    TALANTA, 2021, 224
  • [10] Target-fueled catalytic hairpin assembly for sensitive and multiplex microRNA detection
    Jinyi Wang
    Yue Sun
    Choiwan Lau
    Jianzhong Lu
    Analytical and Bioanalytical Chemistry, 2020, 412 : 3019 - 3027
12345