Response boosting-based approach for absolute quantification of gelatin peptides using LC-MS/MS

被引:6
|
作者
Han, Shuying [1 ,2 ]
Yan, Zhiye [1 ,2 ,3 ]
Huang, Xiaozheng [1 ,2 ,3 ]
Cai, Shuo [1 ,2 ,3 ]
Zhao, Ming [1 ,2 ]
Zheng, Yunfeng [1 ,2 ]
Liu, Xiao [1 ,2 ,4 ]
Xu, Haokun [5 ]
Xie, Yu [5 ]
Hou, Rong [5 ]
Duan, Jin-Ao [1 ,2 ]
Liu, Rui [1 ,2 ,3 ]
机构
[1] Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Chinese Med Resour, Jiangsu Key Lab High Technol Res TCM Formulae, Nanjing 210023, Peoples R China
[2] Nanjing Univ Chinese Med, Sch Pharm, Nanjing 210023, Peoples R China
[3] Nanjing Univ Chinese Med, Jiangsu Key Lab Res & Dev Marine Bio Resource Pha, Nanjing 210023, Peoples R China
[4] Nanjing Univ Chinese Med, Jiangsu Key Lab Chinese Med Proc, Nanjing 210023, Peoples R China
[5] Guizhou Guangjitang Hlth Pharmaceut Co Ltd, Guiyang 550014, Peoples R China
基金
中国国家自然科学基金; 国家重点研发计划;
关键词
Response-boosting; Gelatin peptides; Mass spectrometry; Isotope-labelled internal standard; Absolute quantification; COLLAGEN MARKER PEPTIDES; CHROMATOGRAPHY; IDENTIFICATION; PHARMACEUTICALS; ENHANCEMENT; EXTRACTION; RESIDUES; WATER; MEAT; MS;
D O I
10.1016/j.foodchem.2022.133111
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Response-boosting of MS signal was observed in gelatin samples due to abundant Glycine residues produced by collagen enzymolysis. In this work, a new strategy utilizing response-boosting to enhance detection sensitivity was developed for absolute quantification of Asini Corii Colla, a kind of gelatin commonly used as food therapy products in Asia, by high performance liquid chromatography coupled to tandem mass spectrometry. Peptidomics analysis was used to evaluate the similarity between eight different protein matrices, and deer-hide gelatin was selected as the appropriate simulated matrix. Isotope-labelled internal standard was used to compensate the matrix effect and construct matrix-matched calibration curves. The established method showed reliability in absolute quantification of three species-specific gelatin peptides with good linearity (r(2) > 0.997), precision (RSD < 8.5%), repeatability (RSD < 8.9%), accuracy (recovery 89.4%similar to 106.5%) and sensitivity (LOD 0.02 similar to 0.98 ng/mL). Thus, the present response-boosting based protocol provides a promising application in quality control of food rich in gelatins.
引用
收藏
页数:7
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