Haematological, Biochemical and Therapeutic Aspects of Parturient Haemoglobinuria in Buffaloes

A total of thirty buffaloes suffering from parturient haemoglobinuria were selected from district Chakwal during December 2010 - January 2011. Sixty apparently healthy buffaloes of similar description were also selected from the same areas as controls. Blood samples collected with and without anticoagulant (EDTA) were analyzed for various haematological and biochemical parameters. Significantly (P<0.05) increased mean corpuscular volume (60.72 +/- 8.49fL), mean corpuscular haemoglobin (21.46 +/- 2.52pg), red cell distribution width (16.80 +/- 1.81%), reticutocytes (1.25 +/- 0.66%), platelets (208.13 +/- 85.26x10(3) /mu l) plateletcrit (0.09 +/- 0.04%), mean platelet volume (4.99 +/- 1.85fL), serum alkaline phosphatase (164.20 +/- 88.95u/L), bilimbin conjugated (0.40 +/- 0.17mg/dl), bilirubin unconjugated (0.67 +/- 0.22mg/dl) and bilirubin total (1.07 +/- 0.39mg/dl) whereas; significantly (P<0.05) decreased serum calcium (2.12 +/- 0.11mmoles/L) and phosphorous (2.67 +/- 0.79mg/dL) were recorded in haemoglobinuric buffaloes. compared to healthy controls. Non significant (P>0.05) differences were recorded in mean corpuscular haemoglobin concentration, platelet distribution width, serum glucose, cholesterol and copper concentrations between haemoglobinuric and healthy buffaloes. These thirty haemoglobinuric buffaloes were divided into three groups for clinical trial to compare and assess the recovery rates of three different treatment packages. Highest recovery rate (100%) was recorded for combined therapy of sodium acid phosphate and blood transfusion The recovery rates for combination of sodium acid phosphate with tranexamic acid and tranexamic acid with Novacoc forte injection were 70% and 50%, respectively.