Purification and characterization of a new lectin from the red marine alga Hypnea musciformis

被引：25

作者：

Nagano, CS

Moreno, FBMB

Bloch, C

Prates, MV

Calvete, JJ

Saker-Sampaio, S

Farias, WRL

Tavares, TD

Nascimento, KS

Grangeiro, TB

Cavada, BS

Sampaio, AH

机构：

[1] Univ Fed Ceara, BioMol Lab, Dept Bioquim & Biol Mol, Fortaleza, Ceara, Brazil

[2] EMBRAPA, CENARGEN, Brasilia, DF, Brazil

[3] CSIC, Inst Biomed, E-46010 Valencia, Spain

来源：

PROTEIN AND PEPTIDE LETTERS | 2002年 / 9卷 / 02期

关键词：

D O I：

10.2174/0929866023408931

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

dA lectin from the red marine alga Hypnea musciformis (HML) was purified by extraction with 20 mM PBS, precipitation with 70% saturated ammonium sulphate, ion-exchange DEAE-Cellulose chromatography and RP-HPLC. The 9.3 kDa polypeptide agglutinates erythrocytes from various sources and shows oligomerization. tendencies under certain MALDI-TOF/MS conditions. Preliminary N-terminal sequencing and biological assays strongly suggest that the HML may belong to a new class of algae lectins.

引用

页码：159 / 165

页数：7

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