Purification and characterization of a new lectin from the red marine alga Hypnea musciformis

被引：25
|
作者：
Nagano, CS
Moreno, FBMB
Bloch, C
Prates, MV
Calvete, JJ
Saker-Sampaio, S
Farias, WRL
Tavares, TD
Nascimento, KS
Grangeiro, TB
Cavada, BS
Sampaio, AH
机构：
[1] Univ Fed Ceara, BioMol Lab, Dept Bioquim & Biol Mol, Fortaleza, Ceara, Brazil
[2] EMBRAPA, CENARGEN, Brasilia, DF, Brazil
[3] CSIC, Inst Biomed, E-46010 Valencia, Spain
来源：
PROTEIN AND PEPTIDE LETTERS | 2002年 / 9卷 / 02期
关键词：
D O I：
10.2174/0929866023408931
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
dA lectin from the red marine alga Hypnea musciformis (HML) was purified by extraction with 20 mM PBS, precipitation with 70% saturated ammonium sulphate, ion-exchange DEAE-Cellulose chromatography and RP-HPLC. The 9.3 kDa polypeptide agglutinates erythrocytes from various sources and shows oligomerization. tendencies under certain MALDI-TOF/MS conditions. Preliminary N-terminal sequencing and biological assays strongly suggest that the HML may belong to a new class of algae lectins.
引用
收藏
页码：159 / 165
页数：7
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