Pattern of activation of human antigen presenting cells by genotype GII.4 norovirus virus-like particles

被引：10
|
作者：
Ponterio, Eleonora [1 ]
Petrizzo, Annacarmen [2 ]
Di Bartolo, Ilaria [1 ]
Buonaguro, Franco Maria [2 ]
Buonaguro, Luigi [2 ]
Ruggeri, Franco Maria [1 ]
机构：
[1] Ist Super Sanita, Dept Vet Publ Hlth & Food Safety, I-00161 Rome, Italy
[2] Ist Nazl Studio & Cura Tumori Fdn Pascale IRCCS, Dept Expt Oncol, Lab Mol Biol & Viral Oncol, I-80131 Naples, Italy
来源：
JOURNAL OF TRANSLATIONAL MEDICINE | 2013年 / 11卷
关键词：
Norovirus; Immunology; PBMCs; VLPs; GENE-EXPRESSION PROFILE; DENDRITIC CELLS; MONOCLONAL-ANTIBODIES; IMMUNE-RESPONSES; VACCINE; MUCOSAL; IMMUNOGENICITY; IMMUNIZATION; INFECTION; CYTOKINE;
D O I：
10.1186/1479-5876-11-127
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Background: Virus-like particles (VLPs) from an Italian GII.4 norovirus strain were used to investigate activation and maturation of circulating antigen presenting cells (APCs) of human origin. Methods: Peripheral blood mononuclear cells (PBMCs) isolated from five healthy subjects were pulsed ex vivo with VLPs, and stained with a set of monoclonal antibodies (MAbs) for phenotypic analysis by flow cytometry. Cytokine release in cell supernatants was investigated by ELISA. Results: Norovirus VLPs induced activation and maturation of circulating APCs derived from the five donors, as well as production of IL-6, IFN-gamma and TNF-alpha cytokines. Conclusions: The present results suggest that VLPs can activate antigen presenting cells for an efficient induction of the adaptive immune response.
引用
收藏
页数：7
相关论文
共 50 条
  • [31] Norovirus Vaccine Against Experimental Human GII.4 Virus Illness: A Challenge Study in Healthy Adults
    Bernstein, David I.
    Atmar, Robert L.
    Lyon, G. Marshall
    Treanor, John J.
    Chen, Wilbur H.
    Jiang, Xi
    Vinje, Jan
    Gregoricus, Nicole
    Frenck, Robert W., Jr.
    Moe, Christine L.
    Al-Ibrahim, Mohamed S.
    Barrett, Jill
    Ferreira, Jennifer
    Estes, Mary K.
    Graham, David Y.
    Goodwin, Robert
    Borkowski, Astrid
    Clemens, Ralf
    Mendelman, Paul M.
    JOURNAL OF INFECTIOUS DISEASES, 2015, 211 (06): : 870 - 878
  • [32] Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle Uptake and Presentation by Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo
    Tamminen, Kirsi
    Heinimaeki, Suvi
    Vesikari, Timo
    Blazevic, Vesna
    JOURNAL OF IMMUNOLOGY RESEARCH, 2020, 2020
  • [33] VIRUS-LIKE PARTICLES IN HUMAN LEUKEMIC CELLS
    TORRIOLI, M
    TORRIOLI.G
    BRACONIR.L
    CONFORTI, A
    RUSSO, M
    ACTA GENETICAE MEDICAE ET GEMELLOLOGIAE, 1972, 21 (03): : 271 - 271
  • [34] VIRUS-LIKE PARTICLES IN HUMAN CHLOROLEUKEMIA CELLS
    OTA, Z
    SUZUKI, S
    HIGASHI, S
    GANN, 1963, 54 (04): : 481 - &
  • [35] Adsorption and Aggregation Properties of Norovirus GI and GII Virus-like Particles Demonstrate Differing Responses to Solution Chemistry
    da Silva, Allegra K.
    Kavanagh, Owen V.
    Estes, Mary K.
    Elimelech, Menachem
    ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2011, 45 (02) : 520 - 526
  • [36] Targeting of antigen presenting cells by HIV-1 virus-like particles for transcutaneous vaccination
    Afraz, Z.
    Rancan, F.
    Hadam, S.
    Wagner, R.
    Schaefer-Korting, M.
    Blume-Peytavi, U.
    Vogt, A.
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2013, 133 (05) : 1424 - 1424
  • [37] Chimeric GII.4 Norovirus Virus-Like-Particle-Based Vaccines Induce Broadly Blocking Immune Responses
    Debbink, Kari
    Lindesmith, Lisa C.
    Donaldson, Eric F.
    Swanstrom, Jesica
    Baric, Ralph S.
    JOURNAL OF VIROLOGY, 2014, 88 (13) : 7256 - 7266
  • [38] Parenterally Administered Norovirus GII.4 Virus-Like Particle Vaccine Formulated with Aluminum Hydroxide or Monophosphoryl Lipid A Adjuvants Induces Systemic but Not Mucosal Immune Responses in Mice
    Heinimaki, Suvi
    Malm, Maria
    Vesikari, Timo
    Blazevic, Vesna
    JOURNAL OF IMMUNOLOGY RESEARCH, 2018, 2018
  • [39] Critical role for activation of antigen-presenting cells in priming of cytotoxic T cell responses after vaccination with virus-like particles
    Storni, T
    Lechner, F
    Erdmann, I
    Bächi, T
    Jegerlehner, A
    Dumrese, T
    Kündig, TM
    Ruedl, C
    Bachmann, MF
    JOURNAL OF IMMUNOLOGY, 2002, 168 (06): : 2880 - 2886
  • [40] Characterization of virus-like particles derived from a GII.3 norovirus strain distantly related with current dominating strains
    Huo, Yuqi
    Chen, Xuhui
    Zheng, Lijun
    Huo, Jinling
    Zhang, Shanfeng
    Wang, Mingchen
    Wang, Yumei
    VIRUS GENES, 2016, 52 (05) : 613 - 619
12345