An experiment was carried out to study the effect of sulphur and zinc application on growth, yield attributes, seed yield and quality of mustard at Agronomy Research Farm, College of Agriculture, S. K. Rajasthan Agricultural University, Bikaner (Rajasthan) during rabi season of 2006-2007 and 2007-2008. Treatments consisted of five levels of sulphur (0, 20, 40, 60 and 80 kg/ha) and four levels of zinc (0, 3, 6 and 9 kg/ha). The results indicated that application of 40 kg S/ha and 6 kg Zn/ha improved growth parameters like plant height, number of branches per plant and yield components viz., number of siliquae per plant, seed per siliqua, seed yield and stover yield as compared to other treatments. Oil content in seed increased significantly up to application of 80 kg S/ha. Protein content significantly increased up to 40 kg S/ha and 6 kg Zn/ha.