Electrochemical Fabrication of Prussian Blue Nanocube-decorated Electroreduced Graphene Oxide for Amperometric Sensing of NADH

In this study, Prussian blue (PB) film on the electroreduced graphene oxide (ERGO)-modified Au electrode surface (ERGO/PB) is easily prepared by means of cyclic voltammetric technique in the mixture of K3Fe(CN)(6) and FeCl3. Its electrochemical behaviors for NADH biosensor are studied. The structural and morphological characters of modified electrode material are analyzed with using of XPS, XRD, Raman, EDS, and SEM techniques. ERGO/PB hybrid nanocomposite for NADH biosensor is exhibited to the higher catalytic effect (linear range from 1.0 to 100 mu M, detection limit of 0.23 mu M at S/N=3) compared to naked Au, ERGO-modified Au, and PB-modified Au electrodes. In addition to, ERGO/PB electrode was used to voltammetric and amperometric detection of H2O2. ERGO/PB electrodes also showed the same behavior as the NADH sensor. This ERGO/PB-modified electrode supplied a simple, new, and low-cost route for amperometric sensing of both NADH and H2O2.